Otari Sachin V, Kalia Vipin Chandra, Bisht Aarti, Kim In-Won, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, Seoul 05029, Korea.
Institute of SK-KU Biomaterials, Konkuk University, Seoul 05029, Korea.
Materials (Basel). 2021 Dec 20;14(24):7893. doi: 10.3390/ma14247893.
Metal and metal hybrid nanostructures have shown tremendous application in the biomedical and catalytic fields because of their plasmonic and catalytic properties. Here, a green and clean method was employed for the synthesis of silver nanoparticle (Ag NP)-SiO-FeO hybrid microstructures, and biomolecules from green tea extracts were used for constructing the hybrid structures. The SiO-FeO structures were synthesized using an ethanolic green tea leaf extract to form Bio-SiO-FeO (BSiO-FeO) structures. Biochemical studies demonstrated the presence of green tea biomolecules in the BSiO layer. Reduction of the silver ions was performed by a BSiO layer to form Ag NPs of 5-10 nm in diameter in and on the BSiO-FeO microstructure. The reduction process was observed within 600 s, which is faster than that reported elsewhere. The antimicrobial activity of the Ag-BSiO-FeO hybrid structure was demonstrated against and , and the nanostructures were further visualized using confocal laser scanning microscopy (CLSM). The magnetic properties of the Ag-BSiO-FeO hybrid structure were used for studying reusable antimicrobial activity. Thus, in this study, we provide a novel green route for the construction of a biomolecule-entrapped SiO-FeO structure and their use for the ultra-fast formation of Ag NPs to form antimicrobial active multifunctional hybrid structures.
金属及金属杂化纳米结构因其等离子体和催化特性，在生物医学和催化领域展现出了巨大的应用潜力。在此，我们采用了一种绿色环保的方法来合成银纳米颗粒（Ag NP）-SiO-FeO杂化微结构，并利用绿茶提取物中的生物分子构建该杂化结构。SiO-FeO结构是通过乙醇绿茶叶提取物合成的，从而形成生物SiO-FeO（BSiO-FeO）结构。生化研究表明，BSiO层中存在绿茶生物分子。银离子的还原是由BSiO层完成的，在BSiO-FeO微结构内部及表面形成直径为5-10纳米的Ag NPs。还原过程在600秒内即可完成，这比其他地方报道的速度更快。Ag-BSiO-FeO杂化结构对[具体菌种1]和[具体菌种2]表现出抗菌活性，并且利用共聚焦激光扫描显微镜（CLSM）对纳米结构进行了进一步可视化。Ag-BSiO-FeO杂化结构的磁性被用于研究其可重复使用的抗菌活性。因此，在本研究中，我们提供了一种新颖的绿色途径，用于构建包埋生物分子的SiO-FeO结构，并将其用于超快形成Ag NPs，以形成具有抗菌活性的多功能杂化结构。
