National Heart and Lung Institute, Imperial College London, London, UK
Department of Respiratory Paediatrics, Royal Brompton Hospital, London, UK.
BMJ Open Respir Res. 2021 Dec;8(1). doi: 10.1136/bmjresp-2021-001106.
The airway microbiota is important in chronic suppurative lung diseases, such as primary ciliary dyskinesia (PCD) and cystic fibrosis (CF). This comparison has not previously been described but is important because difference between the two diseases may relate to the differing prognoses and lead to pathological insights and potentially, new treatments.
To compare the longitudinal development of the airway microbiota in children with PCD to that of CF and relate this to age and clinical status.
Sixty-two age-matched children (age range 0.5-17 years) with PCD or CF (n=31 in each group) were recruited prospectively and followed for 1.1 years. Throat swabs or sputum as well as clinical information were collected at routine clinical appointments. 16S rRNA gene sequencing was performed.
The microbiota was highly individual and more diverse in PCD and differed in community composition when compared with CF. While was the most abundant genus in both conditions, was more abundant in CF with more abundant in PCD (P=0.0005). In PCD only, an inverse relationship was seen in the relative abundance of and with age.
Bacterial community composition differs between children with PCD and those with CF. is more prevalent in CF and in PCD, at least until infection with supervenes. Interactions between organisms, particularly members of , and genera appear important. Study of the interactions between these organisms may lead to new therapies or risk stratification.
气道微生物群在慢性化脓性肺部疾病中很重要，例如原发性纤毛运动障碍（PCD）和囊性纤维化（CF）。此前尚未对这两种疾病进行过比较，但这一点很重要，因为两种疾病之间的差异可能与预后不同有关，并能为病理提供见解，并可能带来新的治疗方法。
比较儿童 PCD 与 CF 气道微生物群的纵向发展，并将其与年龄和临床状况相关联。
前瞻性招募了 62 名年龄匹配的 PCD 或 CF 儿童（每组 31 名），并对其进行了为期 1.1 年的随访。在常规临床就诊时采集咽拭子或痰标本以及临床信息。进行 16S rRNA 基因测序。
微生物群高度个体化，PCD 中的多样性更高，与 CF 相比，其群落组成也有所不同。虽然 在两种情况下都是最丰富的属，但 CF 中 更为丰富，而 PCD 中 更为丰富（P=0.0005）。仅在 PCD 中，与年龄呈负相关的是 相对丰度与 。
PCD 和 CF 患儿的细菌群落组成不同。CF 中 更为普遍，而 PCD 中 更为普遍，至少在感染 之前是这样。生物体之间的相互作用，特别是 和 属的成员之间的相互作用似乎很重要。研究这些生物体之间的相互作用可能会带来新的治疗方法或风险分层。
