Ottawa Hospital Research Institute, Neuroscience Program, Ottawa, ON K1H8M5, Canada.
University of Ottawa, Faculty of Medicine, Department of Cellular and Molecular Medicine, Ottawa, ON K1H8M5, Canada.
STAR Protoc. 2021 Dec 11;2(4):101019. doi: 10.1016/j.xpro.2021.101019. eCollection 2021 Dec 17.
Endothelial cells (ECs) lining blood vessels are implicated in organ development, function, and maintenance. We present a detailed protocol enabling isolation and characterization of primary mouse brain ECs, including quality controls and functional assays. These procedures promote survival of primary brain ECs for the assessment of endothelial health. Since alterations in brain ECs are involved in the onset and progression of neurological disorders, this protocol represents a valuable tool to better understand the roles of ECs in brain health. For complete details on the use and execution of this profile, please refer to Ouellette et al. (2020).
血管内皮细胞（ECs）在器官的发育、功能和维持中起着重要作用。我们提供了一个详细的方案，用于分离和鉴定原代小鼠脑 ECs，包括质量控制和功能检测。这些步骤可以促进原代脑 ECs 的存活，用于评估内皮细胞的健康状况。由于脑 ECs 的改变与神经退行性疾病的发生和进展有关，因此该方案代表了一种更好地了解 ECs 在大脑健康中的作用的有价值的工具。有关该方案的使用和实施的完整详细信息，请参阅 Ouellette 等人（2020 年）。
