Department of Industrial Engineering, Bilecik Şeyh Edebali University, Bilecik, Turkey.
Department of Chemical Engineering, Izmir Institute of Technology, Urla, Izmir, Turkey.
J Mater Sci Mater Med. 2021 Dec 23;33(1):6. doi: 10.1007/s10856-021-06632-5.
Hydroxyapatite coated metallic implants favorably combine the required biocompatibility with the mechanical properties. As an alternative to the industrial coating method of plasma spraying with inherently potential deleterious effects, sol-gel methods have attracted much attention. In this study, the effects of intermediate silk fibroin and silk sericin layers on the protein adsorption capacity of hydroxyapatite films formed by a particulate sol-gel method were determined experimentally. The preparation of the layered silk protein/hydroxyapatite structures on glass substrates, and the effects of the underlying silk proteins on the topography of the hydroxyapatite coatings were described. The topography of the hydroxyapatite layer fabricated on the silk sericin was such that the hydroxyapatite particles were oriented forming an oriented crystalline surface. The model protein (bovine serum albumin) adsorption increased to 2.62 µg/cm on the latter surface as compared to 1.37 µg/cm of hydroxyapatite on glass without an intermediate silk sericin layer. The BSA adsorption on glass (blank), glass/c-HAp, glass/m-HAp, glass/sericin/c-HAp, and glass/sericin/m-HAp substrates, reported as decrease in BSA concentration versus contact time.
羟基磷灰石涂层金属植入物将所需的生物相容性与机械性能很好地结合在一起。作为对具有固有有害影响的等离子喷涂工业涂层方法的替代方法，溶胶-凝胶方法引起了广泛关注。在这项研究中，通过颗粒溶胶-凝胶法形成的羟基磷灰石薄膜的蛋白质吸附能力的中间丝素和丝胶层的影响进行了实验确定。描述了在玻璃基底上制备分层丝蛋白/羟基磷灰石结构，以及底层丝蛋白对羟基磷灰石涂层形貌的影响。在丝胶上制备的羟基磷灰石层的形貌使得羟基磷灰石颗粒形成定向结晶表面。与没有中间丝胶层的玻璃上的 1.37μg/cm 的羟基磷灰石相比，模型蛋白（牛血清白蛋白）在后者表面的吸附量增加到 2.62μg/cm。BSA 在玻璃（空白）、玻璃/c-HAp、玻璃/m-HAp、玻璃/丝胶/c-HAp 和玻璃/丝胶/m-HAp 基底上的吸附，报告为 BSA 浓度与接触时间的关系。
