Department of Cardiothoracic Surgery, Zhangzhou Affiliated Hospital of Fujian Medical University, No. 59 Shengli West Road, Zhangzhou, Fujian, 363000, People's Republic of China.
Department of Radiology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian, 363000, People's Republic of China.
Cell Mol Biol Lett. 2021 Dec 24;26(1):54. doi: 10.1186/s11658-021-00298-1.
This study evaluated microRNA-148a-3p in esophageal carcinoma cells. The prediction of bioinformatics analysis revealed that microRNA-148a-3p may target CEP55. qRT-PCR and western blot showed that CEP55 level in esophageal carcinoma cells and tissue was dramatically higher than that of normal cells and tissue, while microRNA-148a-3p was the opposite. Forced expression of microRNA-148a-3p restrained cell malignant behaviors of esophageal carcinoma, and repression of microRNA-148a-3p resulted in the converse results in terms of cell function. Dual-luciferase assay confirmed that microRNA-148a-3p targeted CEP55. CEP55 attenuated the suppressive effect of microRNA-148a-3p on proliferation and migration of esophageal carcinoma cells, demonstrating that microRNA-148a-3p regulated function of esophageal carcinoma cells via decreasing CEP55 level. Microscopy observation indicated that cell morphology was also affected by the microRNA-148a-3p/CEP55 axis. Furthermore, western blot analysis revealed that the PI3K/AKT signaling pathway could be suppressed by activating the microRNA-148a-3p/CEP55 axis. Finally, in vivo experiments confirmed the effects of microRNA-148a-3p on tumorigenesis. Thus, microRNA-148a-3p could act as a repressor in esophageal carcinoma via binding to CEP55.
本研究评估了微小 RNA-148a-3p 在食管癌细胞中的作用。生物信息学分析预测微小 RNA-148a-3p 可能靶向 CEP55。qRT-PCR 和 Western blot 结果显示，食管癌细胞和组织中 CEP55 水平明显高于正常细胞和组织，而微小 RNA-148a-3p 则相反。过表达微小 RNA-148a-3p 抑制了食管癌细胞的恶性行为，而微小 RNA-148a-3p 的抑制则导致细胞功能相反。双荧光素酶报告基因实验证实微小 RNA-148a-3p 靶向 CEP55。CEP55 减弱了微小 RNA-148a-3p 对食管癌细胞增殖和迁移的抑制作用，表明微小 RNA-148a-3p 通过降低 CEP55 水平调节食管癌细胞的功能。显微镜观察表明，细胞形态也受微小 RNA-148a-3p/CEP55 轴的影响。此外，Western blot 分析表明，激活微小 RNA-148a-3p/CEP55 轴可以抑制 PI3K/AKT 信号通路。最后，体内实验证实了微小 RNA-148a-3p 对肿瘤发生的影响。因此，微小 RNA-148a-3p 通过与 CEP55 结合在食管癌中作为一种抑制物发挥作用。
