Mulderry P K, Ghatei M A, Bloom S R
Biochem Biophys Res Commun. 1987 Apr 29;144(2):883-90. doi: 10.1016/s0006-291x(87)80047-5.
Rat tissues contained two forms of calcitonin gene-related peptide (CGRP) immunoreactivity of lower molecular weight than CGRP itself. Two immunoreactive products of in vitro degradation of synthetic CGRP by rat tissue homogenates were purified and shown to be chromatographically identical to these naturally occurring moieties. They reacted only with a carboxy-terminal directed CGRP antiserum indicating that they were carboxy-terminal fragments of CGRP. The larger fragment was found to have a molecular mass corresponding to amino acid residues 19-37 of the CGRP molecule.
大鼠组织中含有两种降钙素基因相关肽（CGRP）免疫反应性物质，其分子量低于CGRP本身。通过大鼠组织匀浆对合成CGRP进行体外降解产生的两种免疫反应性产物被纯化，结果显示它们在色谱上与这些天然存在的部分相同。它们仅与羧基末端定向的CGRP抗血清发生反应，表明它们是CGRP的羧基末端片段。发现较大的片段具有与CGRP分子的氨基酸残基19 - 37相对应的分子量。
