A dual-signal fluorometric and colorimetric sensing platform based on gold-platinum bimetallic nanoclusters for the determination of β-galactosidase activity.

Herein, a novel dual-signal sensing system for the determination of β-galactosidase (β-Gal) activity was established, which was based on a dual-emission probe assembled from gold-platinum bimetallic nanoclusters (Au-Pt NCs) and rhodamine B. Under the catalysis of β-Gal, 4-nitrophenyl β-D-galactopyranoside (PNPG) was rapidly hydrolyzed to generate p-nitrophenol (PNP), which has an obvious UV absorption peak at 400 nm. The hydrolyzed product PNP can quench the fluorescence of Au-Pt NCs effectively by inner filter effect (IFE), and PNP had no impact on the fluorescence of rhodamine B, which will change the emission intensity ratio of Au-Pt NCs and rhodamine B. Therefore, the ratiometric fluorescent and colorimetric dual-signal sensor based on Au-Pt NCs and rhodamine B was successfully constructed for sensitive detection of β-Gal activity. The linear detection range for the ratiometric fluorescence and colorimetric methods were 2.5-25 U/L and 15-55 U/L with detection limits of 1.2 U/L and 5.2 U/L, respectively. The developed assay method has been used for quantitative detection of β-Gal in spiked serum samples and showed good performance. And the detection platform has high reliability and excellent selectivity, which opens a new avenue for the further application of Au-Pt NCs in chemical sensing and biological analysis.