College of Chemistry & Materials Engineering, Wenzhou University, Wenzhou 325035, China.
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Biosensors (Basel). 2023 Aug 23;13(9):838. doi: 10.3390/bios13090838.
Specific identification and monitoring of senescent cells are essential for the in-depth understanding and regulation of senescence-related life processes and diseases. Fluorescent sensors providing real-time and in situ information with spatiotemporal resolution are unparalleled tools and have contributed greatly to this field. This review focuses on the recent progress in fluorescent sensors for molecularly targeted imaging and real-time tracking of cellular senescence. The molecular design, sensing mechanisms, and biological activities of the sensors are discussed. The sensors are categorized by the types of markers and targeting ligands. Accordingly, their molecular recognition and fluorescent performance towards senescence biomarkers are summarized. Finally, the perspective and challenges in this field are discussed, which are expected to assist future design of next-generation sensors for monitoring cellular senescence.
特定识别和监测衰老细胞对于深入了解和调节与衰老相关的生命过程和疾病至关重要。提供具有时空分辨率的实时和原位信息的荧光传感器是无与伦比的工具，为该领域做出了巨大贡献。本综述重点介绍了用于分子靶向成像和实时跟踪细胞衰老的荧光传感器的最新进展。讨论了传感器的分子设计、传感机制和生物活性。根据标记物和靶向配体的类型对传感器进行分类。相应地，总结了它们对衰老生物标志物的分子识别和荧光性能。最后，讨论了该领域的展望和挑战，以期有助于设计用于监测细胞衰老的下一代传感器。
