Junqua S, Wiels J, Tursz T, Le Pecq J B
Immunol Lett. 1987 Jan;14(2):167-72. doi: 10.1016/0165-2478(87)90097-6.
Since immunotoxin (IT) containing the antiglobotriaosylceramide monoclonal antibody was found to be cytotoxic in murine L1210 leukemia cells, its potential antitumor activity could be evaluated in animals using the L1210 model. In vitro, L1210 cells incubated IT before grafting in DBA/2 mice failed to induce leukemia. All tumor cells were neutralized by IT. In animals, a significant but limited therapeutic effect on leukemic mice was obtained when IT was injected i.p. shortly after L1210 cell grafting. In contrast, no toxic effect of IT was observed in non-leukemic mice at doses far above those used in our therapeutic treatment. The potentiation effect of chloroquine on IT was moderated when a cloning efficiency assay was used, but 70% of the mice grafted with in vitro chloroquine-treated L1210 cells were cured with IT treatment.
由于发现含抗球三糖基神经酰胺单克隆抗体的免疫毒素对小鼠L1210白血病细胞具有细胞毒性，因此可以使用L1210模型在动物中评估其潜在的抗肿瘤活性。在体外，将L1210细胞在接种到DBA/2小鼠之前用免疫毒素孵育，未能诱导白血病。所有肿瘤细胞均被免疫毒素中和。在动物中，在L1210细胞接种后不久腹腔注射免疫毒素时，对白血病小鼠获得了显著但有限的治疗效果。相比之下，在远高于我们治疗所用剂量的情况下，未在非白血病小鼠中观察到免疫毒素的毒性作用。当使用克隆效率测定法时，氯喹对免疫毒素的增效作用减弱，但用体外氯喹处理的L1210细胞接种的小鼠中有70%通过免疫毒素治疗治愈。
