Liu Xiaolan, Fu Zhixiao, Zhu Yanfei, Gao Xiwu, Liu Tong-Xian, Liang Pei
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Department of Entomology, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
Pestic Biochem Physiol. 2022 Jan;180:104981. doi: 10.1016/j.pestbp.2021.104981. Epub 2021 Nov 2.
The green peach aphid, Myzus persicae (Sulzer), is a cosmopolitan agricultural pest and causes great damages each year. Afidopyropen is a novel insecticide with high efficacy against even the insecticides resistant M. persicae. However, the sublethal and transgenerational effects of afidopyropen on M. persicae is not clear. In the present paper, sublethal and transgenerational effects of afidopyropen on biological traits of M. persicae were determined based on the age-stage, two-sex life table theory. The afidopyropen was more toxic against M. persicae than other widely used insecticides, with LC of 0.086 mg/L. The treatment with LC, LC and LC concentrations of afidopyropen remarkably reduced the longevity and fecundity of FM. persicae by 15.9-64.4% and 24.3-76.7%, respectively, compared with those of the control. The life history traits of F generation including the pre-adult development time, mean total longevity, pre-adult survival rate, total pre-oviposition period and fecundity were significantly affected after treatment of the F with afidopyropen, and the population parameters, including the net reproductive rate (R), intrinsic rate of increase (r) and finite rate of increase (λ) were also remarkably decreased, while the mean generation time (T) was extended by 6.94%. Among four development and reproduction related genes investigated, JHEH was downregulated by 31.8-38.0% in the afidopyropen treated F generation, while the EcR and JHAMT were overexpressed and the Vg was significantly downregulated in F generation compared to the control group. All these data indicated that the afidopyropen had significant sublethal and transgenerational effects on M. persicae. These results provide insights into comprehensively understanding of the insecticidal effects of afidopyropen on M. persicae as well as the management of resistant M. persicae.
桃蚜（Myzus persicae (Sulzer)）是一种世界性的农业害虫，每年都会造成巨大损失。氟啶虫胺腈是一种新型杀虫剂，对甚至已对杀虫剂产生抗性的桃蚜也具有高效。然而，氟啶虫胺腈对桃蚜的亚致死效应和跨代效应尚不清楚。在本文中，基于年龄-阶段两性生命表理论，测定了氟啶虫胺腈对桃蚜生物学特性的亚致死效应和跨代效应。氟啶虫胺腈对桃蚜的毒性高于其他广泛使用的杀虫剂，其LC₅₀为0.086 mg/L。与对照组相比，用氟啶虫胺腈的LC₁₀、LC₃₀和LC₅₀浓度处理显著降低了桃蚜的寿命和繁殖力，分别降低了15.9 - 64.4%和24.3 - 76.7%。用氟啶虫胺腈处理F₀代后，F₁代的生活史特征，包括成虫前期发育时间、平均总寿命、成虫前期存活率、总产卵前期和繁殖力均受到显著影响，种群参数，包括净繁殖率（R₀）、内禀增长率（r）和周限增长率（λ）也显著降低，而平均世代时间（T）延长了6.94%。在所研究的四个与发育和繁殖相关的基因中，与对照组相比，在氟啶虫胺腈处理的F₁代中，JHEH下调了31.8 - 38.0%，而EcR和JHAMT过表达，Vg显著下调。所有这些数据表明，氟啶虫胺腈对桃蚜具有显著的亚致死效应和跨代效应。这些结果为全面理解氟啶虫胺腈对桃蚜的杀虫作用以及抗性桃蚜的治理提供了见解。
