Guiré Rasmané, Shah Naseer Ali, Meda Roland N T, Ghafoor Hina, Haq Ihsan Ul, Salo Pousbila, Yaseen Aqsa, Al-Asmari Fahad, Zongo Eliasse, Ramadan Mohamed Fawzy, Rizvi Syed Arif Hussain, Turi Shakeel Hussain
Laboratory of Research and Teaching in Animal Health and Biotechnology, Nazi Boni University, Bobo-Dioulasso 1091, Burkina Faso.
Department of Biosciences, COMSTATS University Islamabad, Islamabad 45550, Pakistan.
ACS Omega. 2024 Jul 19;9(30):32799-32806. doi: 10.1021/acsomega.4c03316. eCollection 2024 Jul 30.
The aphid, Rondani (Hemiptera: Aphididae), is one of the most destructive pests of wheat. It is responsible for significant economic losses in the agricultural sector, with an estimated 45% of wheat fields affected. Plant-based insecticides have seen a rapid increase in popularity in recent years due to their efficacy, cost-effectiveness, biodegradability, and lower toxicity compared to synthetic pesticides. The study aimed to evaluate the toxic potential of extracts against and investigate the insect's feeding behavior on wheat. Initially macerated in methanol, the different extracts of organs were fractionated using -hexane, chloroform, ethyl acetate, and butanol. The feeding behavior was analyzed by comparing the waveforms generated by the EPG with the control. After 72 h of treatment, the ethyl acetate fraction extracted from root had the highest toxicity against aphids, with mean 26 mortality of at LC50 of 330 ppm; 25 mortality at LC50 of 400 ppm for leaves; and mean 24.5 mortality at LC50 of 540 ppm in stem bark. EPG analysis indicated that the extract fractions enhanced plant tissue resistance by significantly preventing aphid access to the phloem. The toxic effect of the botanical extracts significantly enhanced the chemical composition of the leaf medium, resulting in a drastic reduction in the number of tissue attacks by . In summary, besides their toxicity to , extracts of reinforce the plant's defense mechanisms, significantly reducing the population. They also reinforce wheat's defense mechanisms. can, therefore, be used as a promising agent in the biological control of .
隆丹尼蚜（半翅目：蚜科）是小麦最具破坏性的害虫之一。它给农业部门造成了巨大的经济损失，估计45%的麦田受到影响。近年来，基于植物的杀虫剂因其有效性、成本效益、生物降解性以及与合成农药相比更低的毒性而迅速受到欢迎。该研究旨在评估[植物名称]提取物对隆丹尼蚜的潜在毒性，并研究该昆虫在小麦上的取食行为。[植物名称]器官的不同提取物最初用甲醇浸渍，然后用正己烷、氯仿、乙酸乙酯和丁醇进行分馏。通过将刺吸电位图谱（EPG）产生的波形与对照进行比较来分析取食行为。处理72小时后，从根部提取的乙酸乙酯馏分对蚜虫的毒性最高，在LC50为330 ppm时平均死亡率为26%；在LC50为400 ppm时叶片死亡率为25%；在LC50为540 ppm时茎皮平均死亡率为24.5%。EPG分析表明，提取物馏分通过显著阻止蚜虫进入韧皮部增强了植物组织的抗性。植物提取物的毒性作用显著改变了叶片介质的化学成分，导致隆丹尼蚜对组织的攻击次数大幅减少。总之，除了对隆丹尼蚜有毒性外，[植物名称]提取物还增强了植物的防御机制，显著减少了隆丹尼蚜的种群数量。它们还增强了小麦的防御机制。因此，[植物名称]可用作隆丹尼蚜生物防治中有前景的药剂。
