Henan Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment, Henan Key Laboratory of Cancer Epigenetics, Cancer Hospital, The First Affiliated Hospital (College of Clinical Medicine) of Henan University of Science and Technology, Luoyang, China.
College of Medicine, Henan University of Science and Technology, Luoyang, China.
Pathol Oncol Res. 2021 Dec 8;27:1609976. doi: 10.3389/pore.2021.1609976. eCollection 2021.
The present study focused on exploring the associations of () infection and low Beclin1 expression with clinicopathological parameters and survival of esophageal squamous cell carcinoma (ESCC) patients, so as to illustrate its clinical significance and prognostic value. Immunohistochemistry (IHC) was used to detect infection status and Beclin1 expression in 370 ESCC patients. The chi-square test was adopted to illustrate the relationship between categorical variables, and Cohen's kappa coefficient was used for correlation analysis. Kaplan-Meier survival curves with the log-rank test were used to analyse the correlation of infection and low Beclin1 expression with survival time. The effects of infection and Beclin1 downregulation on the proliferation, migration and antiapoptotic abilities of ESCC cells were detected by Cell Counting Kit-8, wound healing and flow cytometry assays. For infection of ESCC cells, cell culture medium was replaced with antibiotic-free medium when the density of ESCC cells was 70-80%, cells were inoculated with at a multiplicity of infection (MOI) of 10. infection was negatively correlated with Beclin1 expression in ESCC tissues, and infection and low Beclin1 expression were associated with differentiation status, tumor invasion depth, lymph node metastasis, clinical stage and prognosis in ESCC patients. experiments confirmed that infection and Beclin1 downregulation potentiate the proliferation, migration and antiapoptotic abilities of ESCC cells (KYSE150 and KYSE30). Our results provide evidence that infection and low Beclin1 expression were associated with the development and progression of ESCC. Long-term smoking and alcohol consumption causes poor oral and esophageal microenvironments and ESCC patients with these features were more susceptible to infection and persistent colonization, and exhibited lower Beclin1 expression, worse prognosis and more advanced clinicopathological features. Our findings indicate that effectively eliminating colonization and restoring Beclin1 expression in ESCC patients may contribute to preventation and targeted treatment, and yield new insights into the aetiological research on ESCC.
本研究旨在探讨 感染和低 Beclin1 表达与食管鳞癌 (ESCC) 患者临床病理参数和生存时间的关系，以阐明其临床意义和预后价值。采用免疫组织化学（IHC）检测 370 例 ESCC 患者的 感染状态和 Beclin1 表达。采用卡方检验说明分类变量之间的关系，采用 Cohen's kappa 系数进行相关性分析。采用 Kaplan-Meier 生存曲线和对数秩检验分析 感染和低 Beclin1 表达与生存时间的相关性。通过细胞计数试剂盒-8（Cell Counting Kit-8，CCK-8）、划痕愈合和流式细胞术检测 感染和 Beclin1 下调对 ESCC 细胞增殖、迁移和抗凋亡能力的影响。对于 ESCC 细胞的 感染，当 ESCC 细胞密度为 70-80%时，用无抗生素的培养基替换细胞培养液，以感染复数（multiplicity of infection，MOI）为 10 接种 。ESCC 组织中 感染与 Beclin1 表达呈负相关， 感染和低 Beclin1 表达与 ESCC 患者的分化状态、肿瘤浸润深度、淋巴结转移、临床分期和预后相关。实验证实， 感染和 Beclin1 下调增强了 ESCC 细胞（KYSE150 和 KYSE30）的增殖、迁移和抗凋亡能力。我们的研究结果表明， 感染和低 Beclin1 表达与 ESCC 的发生和发展有关。长期吸烟和饮酒导致口腔和食管微环境恶化，具有这些特征的 ESCC 患者更容易感染 ，并持续定植，表现出较低的 Beclin1 表达、较差的预后和更晚期的临床病理特征。我们的研究结果表明，有效地消除 ESCC 患者的 定植和恢复 Beclin1 表达可能有助于预防和靶向治疗，并为 ESCC 的病因学研究提供新的思路。
