Fuhlbrigge R C, Chaplin D D, Kiely J M, Unanue E R
J Immunol. 1987 Jun 1;138(11):3799-802.
Murine peritoneal exudate cells (PEC), analyzed immediately after isolation, did not express detectable IL 1 activity or IL 1-specific mRNA. Stimulation of these cells by adherence induced the expression of intracellular, membrane, and extracellular IL 1 activities within 4 hr. Analysis of mRNA from these cells showed a concurrent induction of both IL 1 alpha and IL 1 beta mRNA within 1 hr. However, this stimulation of IL 1 expression was transient, since PEC cultured for 5 days no longer expressed IL 1 bioactivity or specific mRNA. Stimulation of these quiescent cells with bacterial lipopolysaccharide induced the re-expression of intracellular, membrane, and extracellular IL 1 activities as well as IL 1 alpha and IL 1 beta mRNA. We found no qualitative difference in the degree or rate of induction of IL 1 alpha compared with IL 1 beta mRNA. These results indicate that resting macrophages are IL 1 negative, and that the IL 1 inducing stimuli used in this study act transiently to increase the levels of IL 1 alpha and IL 1 beta mRNA.
分离后立即分析的小鼠腹腔渗出细胞（PEC）未表达可检测到的白细胞介素1（IL-1）活性或IL-1特异性mRNA。通过贴壁刺激这些细胞可在4小时内诱导细胞内、膜和细胞外IL-1活性的表达。对这些细胞的mRNA分析显示，在1小时内同时诱导了IL-1α和IL-1β mRNA。然而，这种对IL-1表达的刺激是短暂的，因为培养5天的PEC不再表达IL-1生物活性或特异性mRNA。用细菌脂多糖刺激这些静止细胞可诱导细胞内、膜和细胞外IL-1活性以及IL-1α和IL-1β mRNA的重新表达。我们发现与IL-1β mRNA相比，IL-1α的诱导程度或速率没有质的差异。这些结果表明，静息巨噬细胞是IL-1阴性的，并且本研究中使用的IL-1诱导刺激物可短暂起作用以增加IL-1α和IL-1β mRNA的水平。
