Conlon P J, Grabstein K H, Alpert A, Prickett K S, Hopp T P, Gillis S
J Immunol. 1987 Jul 1;139(1):98-102.
The detection and localization of interleukin (IL) 1 in human monocytes was carried out by flow cytometry using monoclonal antibodies to IL-1 alpha and IL-1 beta proteins. IL-1 alpha was detected on the surface of monocytes and the surface expression increased following lipopolysaccharide activation. No demonstrable IL-1 beta protein could be observed on the cell surface by antibody staining, while both IL-1 alpha and IL-1 beta could be visualized intracellularly by the appropriate monoclonal antibodies following acetone permeabilization of the monocytes. Further experiments with cell associated IL-1 revealed that most of the biological activity of human monocytes could be inhibited by affinity purified polyclonal antibodies to IL-1 alpha protein, whereas no inhibitory activity was observed with IL-1 beta specific antibodies. These data support the hypothesis that a differential localization of IL-1 alpha and IL-1 beta exists within human blood-derived monocytes.
利用抗白细胞介素（IL）-1α和IL-1β蛋白的单克隆抗体，通过流式细胞术对人单核细胞中的IL-1进行检测和定位。在单核细胞表面检测到IL-1α，脂多糖激活后其表面表达增加。通过抗体染色在细胞表面未观察到可证实的IL-1β蛋白，而在单核细胞经丙酮通透处理后，用适当的单克隆抗体可在细胞内观察到IL-1α和IL-1β。对细胞相关IL-1的进一步实验表明，人单核细胞的大部分生物活性可被亲和纯化的抗IL-1α蛋白多克隆抗体抑制，而IL-1β特异性抗体未观察到抑制活性。这些数据支持了人血源性单核细胞中存在IL-1α和IL-1β差异定位的假说。
