Saito S, Saito M, Moriyama I, Hino K, Ichijo M
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Apr;39(4):591-8.
Ontogenic development of human NK cells and LAK cells was evaluated in fetuses in spontaneous abortion, premature infants and full term infants. Additionally, we examined the effect of the production of interleukin-2(IL-2) of cord blood lymphocytes on PHA stimulation. The results were as follows. The NK activity of full term infants was significantly lower than that of adults. But augmented NK activity by recombinant IL-2 (20 U/ml) of term infants was the same as adult levels. However, before 32 weeks gestation, both NK activity and augmented NK activity by recombinant IL-2 were lower than that in full term infants. Using two color flow cytometry, after 16 weeks gestation, the percentage of Leu7- Leu11+ cells was almost equal to the adult level, but only a small number of Leu7+ Leu11+ and Leu7+ Leu11- lymphocytes was noted in premature infants and full term infants. LAK activity was evaluated in fetuses. Fetus that had advanced to 19 weeks of gestation had sufficient LAK activity. After 16 weeks gestation, fetal lymphocytes had good IL-2 production. Therefore in infants, LAK cell can play an important role in immunological surveillance against neoplastic tumor.
我们评估了自然流产胎儿、早产儿和足月儿中人类自然杀伤细胞（NK细胞）和淋巴因子激活的杀伤细胞（LAK细胞）的个体发生发育情况。此外，我们还检测了脐血淋巴细胞产生白细胞介素-2（IL-2）对PHA刺激的影响。结果如下。足月儿的NK活性显著低于成年人。但是，足月儿经重组IL-2（20 U/ml）刺激后增强的NK活性与成年人水平相同。然而，在妊娠32周之前，NK活性以及经重组IL-2刺激后增强的NK活性均低于足月儿。采用双色流式细胞术检测发现，妊娠16周后，Leu7-Leu11+细胞的百分比几乎与成年人水平相当，但早产儿和足月儿中仅发现少量Leu7+Leu11+和Leu7+Leu11-淋巴细胞。我们对胎儿的LAK活性进行了评估。妊娠19周的胎儿具有足够的LAK活性。妊娠16周后，胎儿淋巴细胞产生IL-2的能力良好。因此，在婴儿中，LAK细胞在针对肿瘤的免疫监视中可发挥重要作用。
