lncRNA ZFAS1在腰椎间盘退变患者中的预测价值。

Predictive value of lncRNA ZFAS1 in patients with lumbar disc degeneration.

作者信息

Xue Wen, Song Yuxin, Niu Jianxiong, Guan Xiaoli

机构信息

Department of Orthopaedics, Gansu Provincial Hospital Lanzhou 730000, Gansu, China.

The First School of Clinical Medicine, Gansu University of Chinese Medicine Lanzhou 730000, Gansu, China.

出版信息

Am J Transl Res. 2021 Nov 15;13(11):12549-12556. eCollection 2021.

PMID:34956472

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8661246/

Abstract

BACKGROUND

Increasing evidences have indicated the association of non-coding RNAs with the progression of lumbar disc degeneration (LDD), but the role of lncRNA ZFAS1 in LDD remains undefined. Therefore, this study was designed to determine the predictive value of lncRNA ZFAS1 in patients with LDD.

METHODS

A total of 80 patients with LDD confirmed and treated in the Gansu Provincial Hospital from May 2018 to May 2020 were enrolled into the patient group, and 50 healthy controls who concurrently underwent physical examination in our hospital were enrolled into the control group. The expression and diagnostic value of serum lncRNA ZFAS1 in the two groups were determined. The expression of lncRNA ZFAS1 was compared between the two groups before and one month after therapy, and the associations of lncRNA ZFAS1 with inflammatory factors were analyzed. In addition, logistic regression was carried out to analyze risk factors for the prognosis of patients, and corresponding curves about prediction were drawn.

RESULTS

The patient group showed a notably higher lncRNA ZFAS1 level than the control group (P<0.001), and the area under the receiver operating characteristic (ROC) of lncRNA ZFAS1 in diagnosing LDD was 0.807. In addition, after therapy, the patient group showed a remarkable decrease in serum lncRNA ZFAS1 (P<0.01). Serum lncRNA ZFAS1 was positively correlated with serum TNF-α, IL-6 and IL-1β in patients (all P<0.05). Moreover, serum lncRNA ZFAS1 in the good efficacy group was notably lower than that in the general efficacy group (P<0.01). Age and lncRNA ZFAS1 expression before therapy were independent risk factors for patients' prognosis (both P<0.05).

CONCLUSION

LncRNA ZFAS1 is highly expressed in patients with LDD and is a potential prognostic indicator for LDD.

摘要

背景

越来越多的证据表明非编码RNA与腰椎间盘退变（LDD）的进展有关，但lncRNA ZFAS1在LDD中的作用仍不明确。因此，本研究旨在确定lncRNA ZFAS1在LDD患者中的预测价值。

方法

选取2018年5月至2020年5月在甘肃省人民医院确诊并接受治疗的80例LDD患者作为患者组，选取同期在我院进行体检的50例健康对照作为对照组。测定两组血清lncRNA ZFAS1的表达及诊断价值。比较两组治疗前及治疗后1个月lncRNA ZFAS1的表达，并分析lncRNA ZFAS1与炎症因子的相关性。此外，进行逻辑回归分析患者预后的危险因素，并绘制相应的预测曲线。

结果

患者组lncRNA ZFAS1水平显著高于对照组（P<0.001），lncRNA ZFAS1诊断LDD的受试者工作特征曲线（ROC）下面积为0.807。此外，治疗后患者组血清lncRNA ZFAS1显著降低（P<0.01）。患者血清lncRNA ZFAS1与血清TNF-α、IL-6和IL-1β呈正相关（均P<0.05）。此外，疗效良好组血清lncRNA ZFAS1显著低于疗效一般组（P<0.01）。年龄及治疗前lncRNA ZFAS1表达是患者预后的独立危险因素（均P<0.05）。