Zhang Ying, Zou Min, Lodhi Adil Farooq, Deng Yu-Lin
School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Department of Microbiology, Faculty of Biological Sciences, Hazara University, Mansehra, Pakistan.
AMB Express. 2021 Dec 27;11(1):180. doi: 10.1186/s13568-021-01334-1.
Hydroxyl-terminated polybutadiene (HTPB) is a curing adhesive that is commonly used in the production of ammunition, and it emerged during the time of war. After entering the peaceful era, several countries around the globe have focused on the destruction of expired ammunition using safe and economical methods in terms of consumption of energy. Microorganisms exhibit a highly efficient and environment friendly degradation capability for variety of refractory substances. Therefore, in this study we screened five strains of microorganisms from five environmental soil samples for their ability to degrade HTPB. These microorganisms were identified as Microbacterium trichothecenolyticum, Microbacterium esteraromaticum, Arthrobacter pascens, Pseudonocardia carboxydivorans and Ochrobactrum anthropic based on 16S rRNA gene similarity index. We observed the uncorroded and corroded HTPB sample through scanning electron microscopy and observed the formation of lot of holes and gullies in HTPB after corrosion. An 18S rRNA gene clone library was constructed for HTPB-degrading fungi. Based on the results of library evaluation, it was found that the structure of the HTPB-degrading fungi community was relatively simple. A total of 54 positive clones were obtained. These clones represented some uncultured microorganisms that were closely related to Scytalidium lignicola, Pseudokahliella and Gonostomum strenuum. This study will help in the implementation of environment friendly degradation strategies for HTPB degradation.
端羟基聚丁二烯（HTPB）是一种常用于弹药生产的固化粘合剂，它出现在战争时期。进入和平时代后，全球多个国家都致力于采用安全且经济节能的方法销毁过期弹药。微生物对多种难降解物质具有高效且环保的降解能力。因此，在本研究中，我们从五个环境土壤样本中筛选了五株微生物，以评估它们降解HTPB的能力。基于16S rRNA基因相似性指数，这些微生物被鉴定为解单端孢霉微杆菌、酯芳香微杆菌、巴斯德节杆菌、羧基解诺卡氏菌和人苍白杆菌。我们通过扫描电子显微镜观察了未腐蚀和腐蚀后的HTPB样本，发现腐蚀后的HTPB表面形成了许多孔洞和沟壑。构建了一个用于降解HTPB真菌的18S rRNA基因克隆文库。根据文库评估结果，发现降解HTPB真菌群落的结构相对简单。共获得54个阳性克隆。这些克隆代表了一些与木生帚霉、假卡氏菌和强壮嘴口藻密切相关的未培养微生物。本研究将有助于实施针对HTPB降解的环保降解策略。
