Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
Int J Antimicrob Agents. 2022 Feb;59(2):106507. doi: 10.1016/j.ijantimicag.2021.106507. Epub 2021 Dec 25.
Pseudomonas aeruginosa is considered a top priority pathogen associated with elevated morbidity and mortality. Worldwide outbreaks have been associated with a few high-risk epidemic P. aeruginosa lineages. However, the biological features involved in the persistence and spread of such lineages in clinical settings remain to be unravelled. This study reports the emergence of an extensively drug-resistant (XDR) sequence type 309 (ST309) P. aeruginosa in South America (Brazil), specifically in the Amazon region. Genomic analyses were performed with 42 complete and draft ST309 genomes, giving insights into its epidemiology, resistome and mobilome. A heterogeneous distribution of acquired antimicrobial resistance genes among ST309 genomes was observed, which included bla, bla and qnrVC1, all associated with class 1 integrons. Mobilome mining showed the presence of integrative and conjugative elements (ICEs), transposons and genomic islands (GIs) harbouring a huge arsenal of heavy metal resistance determinants, which probably provided adaptive advantages to the ST309 lineage.
铜绿假单胞菌被认为是与发病率和死亡率升高相关的首要病原体。全球范围内的暴发与少数高风险流行的铜绿假单胞菌谱系有关。然而，在临床环境中，这些谱系持续存在和传播所涉及的生物学特征仍有待揭示。本研究报告了在南美洲（巴西），特别是在亚马逊地区出现了广泛耐药（XDR）的序列型 309（ST309）铜绿假单胞菌。对 42 个完整和草案 ST309 基因组进行了基因组分析，深入了解了其流行病学、耐药组和移动组。观察到 ST309 基因组中获得性抗微生物耐药基因的异质性分布，其中包括 blaCTX-M-15、blaOXA-10 和 qnrVC1，均与 1 类整合子有关。移动组挖掘显示存在整合和共轭元件（ICEs）、转座子和基因组岛（GIs），它们携带有大量重金属耐药决定因素，这可能为 ST309 谱系提供了适应性优势。
