Sasaki Minoru, Shimoyama Yu, Kodama Yoshitoyo, Ishikawa Taichi
Department of Microbiology, Division of Molecular Microbiology, Iwate Medical University, Morioka 028-3694, Japan.
Pathogens. 2021 Dec 20;10(12):1648. doi: 10.3390/pathogens10121648.
is the most common microorganism associated with adult periodontal disease, causing inflammation around the subgingival lesion. In this study, we investigated tryptophanyl tRNA synthase (WRS) production by THP-1 cells infected with . Cytokine production, leukocyte adhesion molecules, and low-density lipoprotein receptor (LDLR) expressions in cultured cells were examined. WRS was detected in THP-1 cell culture supernatants stimulated with from 1 to 24 h, and apparent production was observed after 4 h. No change in WRS mRNA expression was observed from 1 to 6 h in THP-1 cells, whereas its expression was significantly increased 12 h after stimulation with . Lactate dehydrogenase (LDH) activity was observed from 4 to 24 h. The TNF-α, IL-6, IL-8, and CXCL2 levels of THP-1 cells were upregulated after treatment with recombinant WRS (rWRS) and were significantly reduced when THP-1 cells were treated with C29. The MCP-1, ICAM-1, and VCAM-1 levels in human umbilical vein endothelial cells were upregulated following treatment with rWRS, and TAK242 suppressed these effects. Additionally, unmodified LDLR, macrophage scavenger receptor A, and lectin-like oxidized LDLRs were upregulated in THP-1 cells treated with rWRS. These results suggest that WRS from macrophages infected with is associated with atherosclerosis.
是与成人牙周病相关的最常见微生物，可导致龈下病变周围的炎症。在本研究中，我们调查了感染 的THP-1细胞产生色氨酰tRNA合成酶（WRS）的情况。检测了培养细胞中的细胞因子产生、白细胞粘附分子和低密度脂蛋白受体（LDLR）表达。在用 刺激THP-1细胞培养上清液1至24小时后检测到WRS，4小时后观察到明显产生。在THP-1细胞中，从1至6小时未观察到WRS mRNA表达的变化，而在用 刺激12小时后其表达显著增加。在4至24小时观察到乳酸脱氢酶（LDH）活性。用重组WRS（rWRS）处理后，THP-1细胞的TNF-α、IL-6、IL-8和CXCL2水平上调，而用C29处理THP-1细胞时这些水平显著降低。用rWRS处理后人脐静脉内皮细胞中的MCP-1、ICAM-1和VCAM-1水平上调，TAK242抑制了这些作用。此外，在用rWRS处理的THP-1细胞中，未修饰的LDLR、巨噬细胞清道夫受体A和凝集素样氧化LDLR上调。这些结果表明，感染 的巨噬细胞产生的WRS与动脉粥样硬化有关。
