Hsu André Keng Wei, Roman Silvane Souza, Bagatini Margarete Dulce, Marafon Filomena, do Nascimento Junior Paulo, Modolo Norma Sueli Pinheiro
Anesthesia Department, São Paulo State University (UNESP)-Botucatu, Botucatu 18618683, Brazil.
Pharmacology and Histology Department, Medical School, Integrated Regional University (URI), Erechim 99709910, Brazil.
Nutrients. 2021 Dec 17;13(12):4519. doi: 10.3390/nu13124519.
(1) Background: Intermittent fasting is a nutrition practice in which individuals fast for several hours in a day, mainly with feeding time during the daylight hours. They seek to improve metabolic performance and cellular resistance to stress. In this study, we tested the fasting protocol to investigate the glycemic effect in a laparotomy perioperative period in diabetic rats and histopathologic findings. (2) Methods: The animals were diabetic-induced with alloxan. Two groups were set according to the feeding protocol: free food and intermittent fasting, whose rats could only eat 8 h in the daylight. Both groups were anesthetized, and a laparotomy was performed. We evaluated the glucose levels during the perioperative period, and we accessed organ histology seeking damage of kidney, bowel and liver after surgical trauma, and we evaluated the wound healing process. (3) Results: Glycemic levels were improved in the intermittent fasting group, especially in the post-operative period after laparotomy. Comparing both groups' tubular damage showed interdependency with mice with worse glycemic level (Z = 2.3; = 0.0215) and wound-healing parameters showed interdependency with rats with better glycemic status for neovascularization (Z = 2.2; = 0.0273) and the presence of sebaceous and sweat gland in the healing process (Z = 2.30; = 0.0215). (4) Conclusions: Intermittent fasting before surgery can be a tool to improve glycemic levels in diabetic rats, with improvement especially in the post-operative period.
(1) 背景：间歇性禁食是一种营养实践方式，个体在一天中禁食数小时，主要进食时间集中在白天。他们试图改善代谢功能以及细胞对压力的抵抗力。在本研究中，我们测试了禁食方案，以调查其对糖尿病大鼠剖腹手术围手术期血糖的影响以及组织病理学结果。(2) 方法：用四氧嘧啶诱导动物患糖尿病。根据进食方案分为两组：自由进食组和间歇性禁食组，间歇性禁食组的大鼠仅在白天8小时内进食。两组均进行麻醉并实施剖腹手术。我们评估了围手术期的血糖水平，观察了手术创伤后肾脏、肠道和肝脏的器官组织学损伤情况，并评估了伤口愈合过程。(3) 结果：间歇性禁食组的血糖水平有所改善，尤其是在剖腹手术后的时期。比较两组的肾小管损伤情况发现，其与血糖水平较差的小鼠存在相关性（Z = 2.3；P = 0.0215），而伤口愈合参数与血糖状态较好的大鼠在新生血管形成方面存在相关性（Z = 2.2；P = 0.0273），并且在愈合过程中皮脂腺和汗腺的存在情况也与血糖状态较好的大鼠存在相关性（Z = 2.30；P = 0.0215）。(4) 结论：术前间歇性禁食可能是改善糖尿病大鼠血糖水平的一种手段，尤其在术后阶段效果更佳。
