Department of Chemical & Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235, USA.
Interdisciplinary Material Science Program, Vanderbilt University, Nashville, TN 37235, USA.
Sensors (Basel). 2021 Dec 10;21(24):8248. doi: 10.3390/s21248248.
The detection of pathogens presents specific challenges in ensuring that biosensors remain operable despite exposure to elevated temperatures or other extreme conditions. The most vulnerable component of a biosensor is typically the bioreceptor. Accordingly, the robustness of peptides as bioreceptors offers improved stability and reliability toward harsh environments compared to monoclonal antibodies that may lose their ability to bind target molecules after such exposures. Here, we demonstrate peptide-based capture of the Chikungunya virus E2 protein in a porous silicon microcavity biosensor at room temperature and after exposure of the peptide-functionalized biosensor to high temperature. Contact angle measurements, attenuated total reflectance-Fourier transform infrared spectra, and optical reflectance measurements confirm peptide functionalization and selective E2 protein capture. This work opens the door for other pathogenic biomarker detection using peptide-based capture agents on porous silicon and other surface-based sensor platforms.
病原体的检测存在一些特殊的挑战，需要确保生物传感器在暴露于高温或其他极端条件下仍能正常运行。生物传感器中最脆弱的组件通常是生物感受器。因此，与单克隆抗体相比，肽作为生物感受器具有更好的稳定性和可靠性，单克隆抗体在暴露后可能会失去与靶分子结合的能力。在这里，我们展示了在室温下以及在肽功能化生物传感器暴露于高温后，在多孔硅微腔生物传感器中基于肽的寨卡病毒 E2 蛋白的捕获。接触角测量、衰减全反射傅里叶变换红外光谱和光反射测量证实了肽的功能化和对 E2 蛋白的选择性捕获。这项工作为使用基于肽的捕获剂在多孔硅和其他基于表面的传感器平台上进行其他致病生物标志物的检测开辟了道路。
