Beckner S K, Farrar W L
Biochem Biophys Res Commun. 1987 May 29;145(1):176-82. doi: 10.1016/0006-291x(87)91303-9.
Interleukin 2 (IL 2) inhibited basal as well as PGE2, isoproterenol and forskolin stimulated cAMP production in human T lymphocytes. Although the stimulation of adenylate cyclase by activators of the enzyme was evident in lymphocyte membrane preparations, the inhibitory effect of IL 2 was observed only if cells were pretreated with IL 2 and the membranes activated with Ca++ and ATP. Additionally, when purified protein kinase C was reconstituted into untreated membranes and activated with Ca++ and ATP, both receptor and non-receptor stimulated adenylate cyclase was inhibited. These results suggest that the inhibition of adenylate cyclase in human T lymphocytes by IL 2 is mediated by protein kinase C.
白细胞介素2（IL-2）可抑制人T淋巴细胞中基础状态下以及前列腺素E2、异丙肾上腺素和毛喉素刺激的环磷酸腺苷（cAMP）生成。尽管在淋巴细胞膜制剂中该酶的激活剂对腺苷酸环化酶的刺激作用很明显，但IL-2的抑制作用只有在细胞先用IL-2预处理且膜用钙离子（Ca++）和三磷酸腺苷（ATP）激活后才会观察到。此外，当将纯化的蛋白激酶C重组到未处理的膜中并用Ca++和ATP激活时，受体介导和非受体介导刺激的腺苷酸环化酶均受到抑制。这些结果表明，IL-2对人T淋巴细胞中腺苷酸环化酶的抑制作用是由蛋白激酶C介导的。
