Gommans J M, van Hezik S J, van Huystee B E
Br J Dermatol. 1987 May;116(5):661-6. doi: 10.1111/j.1365-2133.1987.tb05899.x.
We describe a flow cytometric technique for measuring the percentage of T6-positive cells in suspensions prepared by trypsinization of human epidermis. The value obtained for healthy controls (1.55 +/- 0.52%) corresponds to about 600 T6-positive cells per mm2 of skin surface, a figure in line with histological estimates. No significant change was found in the percentage of T6-positive cells in either the uninvolved or lesional epidermis of untreated psoriatic patients. PUVA treatment resulted in a significant reduction in T6-positive cells. A cyclic fluctuation in the numbers of T6-positive cells was shown to accompany methotrexate administration on a weekly divided dose schedule.
我们描述了一种流式细胞术技术，用于测量通过胰蛋白酶消化人表皮制备的悬浮液中T6阳性细胞的百分比。健康对照获得的值（1.55±0.52%）相当于每平方毫米皮肤表面约600个T6阳性细胞，这一数字与组织学估计相符。在未经治疗的银屑病患者的非病变或病变表皮中，T6阳性细胞的百分比均未发现显著变化。补骨脂素紫外线A（PUVA）治疗导致T6阳性细胞显著减少。结果显示，每周分次给药甲氨蝶呤时，T6阳性细胞数量会出现周期性波动。
