艾顿叶提取物抑制3T3-L1脂肪细胞中的脂质积累和尿酸生成。

Aiton Leaves Extract Suppressed Lipid Accumulation and Uric Acid Production in 3T3-L1 Adipocytes.

作者信息

Yamasaki Masao, Kiue Yusei, Fujii Kento, Sushida Moe, Yamasaki Yumi, Sugamoto Kazuhiro, Suzuki Yosuke, Koga Yasuko, Kunitake Hisato, Kai Hisahiro, Ogawa Kenjiro, Nishiyama Kazuo, Goto Yo, Nakayama Takayuki

机构信息

Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.

Faculty of Regional Innovation, University of Miyazaki, Miyazaki 889-2192, Japan.

出版信息

Plants (Basel). 2021 Nov 30;10(12):2638. doi: 10.3390/plants10122638.

DOI:10.3390/plants10122638

PMID:34961109

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8705443/

Abstract

Blueberry ( Aiton; Kinisato 35 Gou) leaves have recently attracted increasing attention as a useful material for the prevention of lifestyle diseases. Here, we examined the effects of the hot water extract of blueberry leaves (BLEx) on lipogenesis and uric acid production in 3T3-L1 adipocytes. The results showed that BLEx suppressed lipid accumulation and the mRNA expression of differentiation markers in 3T3-L1 adipocytes. A fractionation study showed that the highly polymerized proanthocyanidin-rich fraction was responsible for this effect. Upon maturation to adipocytes, 3T3-L1 cells produced uric acid and tumor necrosis factor-α, and hypoxia stimulated the production of uric acid and xanthine oxidoreductase activity. BLEx suppressed the production of uric acid under these conditions. Although BLEx inhibited the enzymatic activity of xanthine oxidase, this activity was observed in several fractions containing catechin, epicatechin, chlorogenic acid, rutin, and low molecular weight proanthocyanidins. Taken together, these results indicate that BLEx contains various compounds with the ability to suppress lipid accumulation and uric acid production in adipocytes.

摘要

蓝莓（艾顿；纪之里35号）叶作为预防生活方式疾病的有用材料，最近受到了越来越多的关注。在此，我们研究了蓝莓叶热水提取物（BLEx）对3T3-L1脂肪细胞脂质生成和尿酸产生的影响。结果表明，BLEx抑制了3T3-L1脂肪细胞中的脂质积累和分化标志物的mRNA表达。分级分离研究表明，高度聚合的富含原花青素的级分是造成这种效应的原因。3T3-L1细胞成熟为脂肪细胞后会产生尿酸和肿瘤坏死因子-α，缺氧会刺激尿酸的产生和黄嘌呤氧化还原酶活性。在这些条件下，BLEx抑制了尿酸的产生。虽然BLEx抑制了黄嘌呤氧化酶的酶活性，但在含有儿茶素、表儿茶素、绿原酸、芦丁和低分子量原花青素的几个级分中都观察到了这种活性。综上所述，这些结果表明，BLEx含有多种能够抑制脂肪细胞中脂质积累和尿酸产生的化合物。