Letaief Touka, Garzoli Stefania, Laghezza Masci Valentina, Mejri Jamel, Abderrabba Manef, Tiezzi Antonio, Ovidi Elisa
Laboratory of Materials Molecules and Applications (LMMA), IPEST, University of Carthage, BP 51, La Marsa 2070, Tunisia.
National Agronomic Institute of Tunisia (INAT), University of Carthage, Mahrajene 1082, Tunisia.
Plants (Basel). 2021 Dec 2;10(12):2651. doi: 10.3390/plants10122651.
The Tunisian plant was investigated to determine its phytoconstituents and evaluate its biological activities. In particular, the GC/MS technique was used to describe the chemical composition of active extracts and fractions. Among the obtained extracts, the yields of the dried root methanolic extract (29.80%) and the fruit aqueous extract (48.00%) were the highest ones. The dried root methanolic extract exhibited the highest amount in the total phenolics (186.44 ± 0.26 mg GAE/g DW), total flavonoids (102.50 ± 3.53 mg QE/g DW), and tannins (60.714 ± 2.2 mg catechin/g DW). The root aqueous extracts revealed the highest antioxidant activity with an IC of 8.96 ± 0.38 mg/L and 16.46 ± 0.60 mg/L for the ABTS and DPPH• assays, respectively. The total antioxidant capacity was accorded to the methanolic extract of the dried roots with a value of 304.07 ± 1.11 µg AAE/mg. The drying process was found to improve the qualitative and quantitative properties of the extracts. The evaluation of the cytotoxic activity against the SH-SY5Y cell line was carried out using MTT assay. The petroleum ether and dichloromethane extracts of the dried roots showed relevant cytotoxic activities. The thin layer chromatography and the GC-MS/GC-FID analysis led to the identification of the 13-epimanool as a potent cytotoxic compound.
对突尼斯的这种植物进行了研究，以确定其植物成分并评估其生物活性。特别是，采用气相色谱/质谱联用技术描述活性提取物和馏分的化学成分。在所获得的提取物中，干燥根甲醇提取物（29.80%）和果实水提取物（48.00%）的产率最高。干燥根甲醇提取物的总酚含量（186.44±0.26毫克没食子酸当量/克干重）、总黄酮含量（102.50±3.53毫克槲皮素当量/克干重）和单宁含量（60.714±2.2毫克儿茶素/克干重）最高。根水提取物在ABTS和DPPH• 测定中的抗氧化活性最高，IC50分别为8.96±0.38毫克/升和16.46±0.60毫克/升。干燥根的甲醇提取物的总抗氧化能力为304.07±1.11微克抗坏血酸当量/毫克。发现干燥过程可改善提取物的定性和定量性质。使用MTT法对SH-SY5Y细胞系进行细胞毒性活性评估。干燥根的石油醚提取物和二氯甲烷提取物显示出相关的细胞毒性活性。薄层色谱法和气相色谱-质谱联用/气相色谱-氢火焰离子化检测分析鉴定出13-表曼诺醇是一种有效的细胞毒性化合物。
