Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, Viterbo, Italy.
Department of Drug Chemistry and Technology, Sapienza University, Rome, Italy.
Nat Prod Res. 2021 Jun;35(11):1923-1927. doi: 10.1080/14786419.2019.1644628. Epub 2019 Jul 22.
The aim of this study is to define chemical composition and antiproliferative activity of several compounds isolated from male and female flowers on human neuroblastoma (SH-SY5Y) and human leukemia (HL60) cell lines. Three extracts obtained by using solvents with different polarities (petroleum ether, diethyl ether and acetone) were characterized by GC/MS analysis revealing qualitative and quantitative differences in the chemical composition of male and female flowers. Nineteen components were identified and both flower extracts were dominated by the sesquiterpene hydrocarbons fraction, mainly elemol, followed by bicyclic sesquiterpenes and by a smaller amount of monoterpenes. The biological activities of the extracts were evaluated by dose-dependent viability assay on two different cell lines and HL60 cells resulted more sensitive to the treatment. The presence of compounds at low concentration with a well-established cytotoxic activity such as β-elemene, β-caryophyllene and γ-eudesmol could exert a synergistic cytotoxic effect.
本研究旨在定义从雄花和雌花中分离得到的几种化合物的化学成分和抗增殖活性，这些化合物用于人类神经母细胞瘤（SH-SY5Y）和人白血病（HL60）细胞系。使用不同极性的溶剂（石油醚、乙醚和丙酮）获得了三种提取物，通过 GC/MS 分析对其进行了表征，揭示了雄花和雌花化学成分的定性和定量差异。鉴定出 19 种成分，两种花提取物均以倍半萜类碳氢化合物为主，主要为艾醇，其次是双环倍半萜类和少量单萜类。通过对两种不同细胞系的剂量依赖性活力测定评估了提取物的生物活性，HL60 细胞对治疗更为敏感。低浓度下存在具有明确细胞毒性的化合物，如 β-榄香烯、β-石竹烯和γ-桉叶醇，可能发挥协同细胞毒性作用。
