Blinstrubienė Aušra, Jančauskienė Inga, Burbulis Natalija
Department of Plant Biology and Food Sciences, Vytautas Magnus University Agriculture Academy, Donelaicio str. 58, 44248 Kaunas, Lithuania.
Plants (Basel). 2021 Dec 17;10(12):2799. doi: 10.3390/plants10122799.
x is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of x and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L IBA + 0.1 mg L BAP + 4.0 mg L l-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L ZEA + 1.0 mg L NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L IBA, where more than 95% of plant regenerants survived and were growing normally.
x是一种自发的不育杂种，因此通过传统育种方法创造有用的遗传多样性受到限制。通过间接器官发生进行植株再生可能是创造这种重要农作物遗传变异的一种有用方法。本研究旨在评估外植体类型和生长调节剂对x间接器官发生的影响，并通过流式细胞术确定植株再生体的倍性水平。平均而言，在添加2.5 mg/L吲哚丁酸（IBA）+ 0.1 mg/L苄氨基嘌呤（BAP）+ 4.0 mg/L L-脯氨酸的培养基中，测试外植体形成的形态发生愈伤组织百分比最高。在添加4.0 mg/L玉米素（ZEA）+ 1.0 mg/L萘乙酸（NAA）的培养基中，观察到愈伤组织细胞最强烈的二次分化。在添加0.4 mg/L IBA的MS营养培养基中，确定了最高的生根频率和最多的根数，超过95%的植株再生体存活并正常生长。
