Xu Xiaoyu, Deng Wen, Zhang Wanqing, Zhang Junhua, Wang Muchen, Shan Si, Liu Hongning
Jiangxi Province Key Laboratory of TCM Etiopathogenesis, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China.
Research Center for Differentiation and Development of TCM Basic Theory, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China.
Evid Based Complement Alternat Med. 2021 Dec 18;2021:5107441. doi: 10.1155/2021/5107441. eCollection 2021.
The increasing use of moxibustion has led to a debate concerning the safety of this treatment in human patients. Inhalation of cigarette smoke induces lung inflammation and granulomas, the proliferation of alveolar epithelial cells, and other toxic effects; therefore, it is important to assess the influence of inhaled moxa smoke on the lungs. In the present study, a novel poisoning cabinet was designed and used to assess the acute toxicity of moxa smoke in rats. We evaluated pathological changes in rat lung tissue and analyzed differentially expressed genes (DEGs) using RNA-seq and transcriptomic analyses. Our results show that the maximum tolerable dose of moxa smoke was 290.036 g/m³ and LC was 537.65 g/m³. Compared with that of the control group, the degree of inflammatory cell infiltration in the lung tissues of group A rats (all dead group) was increased, while that in group E rats (all live group) remained unchanged. GO and KEGG enrichment analyses showed that the DEGs implicated in cell components, binding, and cancer were significantly enriched in the experimental groups compared with the profile of the control group. The expressions of , , , , and determined using quantitative real-time PCR were similar to those determined using RNA-seq, confirming the reliability of RNA-seq data. Overall, our results provide a basis for future evaluations of moxibustion safety and the development of moxibustion-based technology.
艾灸使用的日益增加引发了关于这种治疗方法对人类患者安全性的争论。吸入香烟烟雾会引发肺部炎症和肉芽肿、肺泡上皮细胞增殖以及其他毒性作用；因此，评估吸入艾烟对肺部的影响很重要。在本研究中，设计了一种新型中毒柜并用于评估艾烟对大鼠的急性毒性。我们评估了大鼠肺组织的病理变化，并使用RNA测序和转录组分析来分析差异表达基因（DEGs）。我们的结果表明，艾烟的最大耐受剂量为290.036 g/m³，致死浓度为537.65 g/m³。与对照组相比，A组大鼠（全部死亡组）肺组织中的炎症细胞浸润程度增加，而E组大鼠（全部存活组）的炎症细胞浸润程度保持不变。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，与对照组相比，实验组中涉及细胞成分、结合和癌症的差异表达基因显著富集。使用定量实时PCR测定的 、 、 、 和 的表达与使用RNA测序测定的结果相似，证实了RNA测序数据的可靠性。总体而言，我们的结果为未来艾灸安全性评估和基于艾灸的技术开发提供了依据。
