在中国甘肃省六个不同自然景观地区的动物宿主和媒介中检测和基因分型。

Detection and Genotyping of in Animal Hosts and Vectors from Six Different Natural Landscape Areas, Gansu Province, China.

机构信息

Faculty of Medicine, Northwest Minzu University, Lanzhou 730030, China.

Key Laboratory of Environmental Ecology and Population Health in Northwest Minority Areas, Northwest Minzu University, Lanzhou 730030, China.

出版信息

Comput Math Methods Med. 2021 Dec 18;2021:6820864. doi: 10.1155/2021/6820864. eCollection 2021.

DOI:10.1155/2021/6820864

PMID:34961822

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8710147/

Abstract

OBJECTIVE

Tularemia, also known as hare fever, is caused by the bacterium () transmitted through diseased wild animals, blood sucking insects, or contaminated water or food, which is distributed worldwide. The purpose of this study was to investigate infection in animal hosts and vectors from six different natural landscape areas in Gansu Province and to identify the genotypes of the detected .

METHODS

Rodents were captured by snap traps, and ticks were collected by dragging a cloth over the vegetation or from domestic animals. After species identification, DNA was isolated from the captured animals and detected by nested PCR assays targeting the fopA gene. The positive samples were further amplified to discriminate the species, and another two short-sequence tandem repeat regions (SSTR) were amplified to identify their genotypes. All positive fragments were sequenced and analyzed by ClustalX (5.0) and DNAClub software.

RESULTS

A total of 407 rodents of 12 species were captured, among which six rodent species were positive for , with an overall prevalence of 3.93%. The geographical difference in infection rate was statistically significant. At the SSTR9 locus, there were 7 genotypes among positive rodent samples. A total of 1864 ticks were tested for evidence of tularemia by nested PCR assays, 69 of which were positive, with an average positive rate of 3.70% for in ticks. The positive rates were significantly different among different regions. Seven genotypes were identified at the SSTR9 locus, one of which seemed dominant in positive tick samples. All positive samples had the same genotype at the SSTR16 locus.

CONCLUSION

There is natural infection of among animal vectors and hosts in Gansu Province, with diverse genotypes.

摘要

目的

土拉菌病又称野兔热，是由（）细菌引起的，通过患病野生动物、吸血昆虫或受污染的水或食物传播，分布于世界各地。本研究旨在调查甘肃省六个不同自然景观地区动物宿主和媒介中的感染情况，并鉴定所检测到的基因型。

方法

用捕鼠夹捕获啮齿动物，用布条在植被上或从家畜身上拖过收集蜱虫。在鉴定物种后，从捕获的动物中提取 DNA，并用针对 fopA 基因的巢式 PCR 检测方法进行检测。对阳性样本进行进一步扩增以区分物种，并扩增另外两个短串联重复区（SSTR）以鉴定其基因型。所有阳性片段均进行测序，并通过 ClustalX（5.0）和 DNAClub 软件进行分析。

结果

共捕获 407 只 12 种啮齿动物，其中 6 种啮齿动物呈阳性，总体感染率为 3.93%。感染率的地理差异具有统计学意义。在 SSTR9 基因座上，阳性鼠样本中有 7 种基因型。用巢式 PCR 检测方法对 1864 只蜱虫进行了土拉菌病检测，其中 69 只为阳性，蜱虫中的阳性率为 3.70%。不同地区的阳性率差异有统计学意义。在 SSTR9 基因座上鉴定出 7 种基因型，其中一种在阳性蜱虫样本中似乎占优势。所有阳性样本在 SSTR16 基因座上具有相同的基因型。