Pharmacy School, Jinzhou Medical University, Jinzhou, China.
Department of Emergency Management, Liaoning Provincial Center for Disease Control and Prevention, Shenyang, China.
Drug Deliv. 2022 Dec;29(1):31-42. doi: 10.1080/10717544.2021.2015481.
In this study, we prepared a folic acid-functionalized SMMC-7721 liver cancer cell membrane (CM)-encapsulated paclitaxel nanocrystals system (FCPN) for hepatoma treatment. Transmission electron microscopy (TEM) characterization showed that FCPN was irregular spherical shapes with a particle size larger than 200 nm and a coated thickness of approximately 20 nm. In an release experiment, FCPN indicated a slowly release effect of paclitaxel (PTX). Cell experiments demonstrated that FCPN was taken up by SMMC-7721 cells and significantly inhibited the proliferation of SMMC-7721 cells, which illustrated that FCPN had good targeting ability compared with PN and CPN. According to the results of animal experiments, FCPN significantly inhibited tumor growth. Tissue distribution experiments proved that FCPN could accumulate significantly in tumor tissues, which further explained why FCPN had good targeting ability. These results clearly suggested that folate-functionalized homotypic CM bionic nanosystems might represent a very valuable method for liver cancer treatment in the future.
在本研究中，我们制备了叶酸功能化 SMMC-7721 肝癌细胞膜（CM）包裹紫杉醇纳米晶系统（FCPN）用于肝癌治疗。透射电子显微镜（TEM）表征显示，FCPN 呈不规则球形，粒径大于 200nm，包覆厚度约为 20nm。在释放实验中，FCPN 显示出紫杉醇（PTX）的缓慢释放效果。细胞实验表明，FCPN 被 SMMC-7721 细胞摄取，并显著抑制 SMMC-7721 细胞的增殖，这表明 FCPN 与 PN 和 CPN 相比具有良好的靶向能力。根据动物实验的结果，FCPN 显著抑制了肿瘤的生长。组织分布实验证明 FCPN 可以在肿瘤组织中显著聚集，这进一步解释了为什么 FCPN 具有良好的靶向能力。这些结果清楚地表明，叶酸功能化同源 CM 仿生纳米系统可能代表未来肝癌治疗的一种非常有价值的方法。
