莫拉克斯氏菌属为主的儿童鼻咽微生物群与上呼吸道感染和鼻窦炎相关。
Moraxella-dominated pediatric nasopharyngeal microbiota associate with upper respiratory infection and sinusitis.
作者信息
McCauley Kathryn E, DeMuri Gregory, Lynch Kole, Fadrosh Douglas W, Santee Clark, Nagalingam Nabeetha N, Wald Ellen R, Lynch Susan V
机构信息
Division of Gastroenterology, Department of Medicine, University of California San Francisco, San Francisco, CA, United States of America.
Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States of America.
出版信息
PLoS One. 2021 Dec 28;16(12):e0261179. doi: 10.1371/journal.pone.0261179. eCollection 2021.
BACKGROUND
Distinct bacterial upper airway microbiota structures have been described in pediatric populations, and relate to risk of respiratory viral infection and, exacerbations of asthma. We hypothesized that distinct nasopharyngeal (NP) microbiota structures exist in pediatric populations, relate to environmental exposures and modify risk of acute sinusitis or upper respiratory infection (URI) in children.
METHODS
Bacterial 16S rRNA profiles from nasopharyngeal swabs (n = 354) collected longitudinally over a one-year period from 58 children, aged four to seven years, were analyzed and correlated with environmental variables, URI, and sinusitis outcomes.
RESULTS
Variance in nasopharyngeal microbiota composition significantly related to clinical outcomes, participant characteristics and environmental exposures including dominant bacterial genus, season, daycare attendance and tobacco exposure. Four distinct nasopharyngeal microbiota structures (Cluster I-IV) were evident and differed with respect to URI and sinusitis outcomes. These clusters were characteristically either dominated by Moraxella with sparse underlying taxa (Cluster I), comprised of a non-dominated, diverse microbiota (Cluster II), dominated by Alloiococcus/Corynebacterium (Cluster III), or by Haemophilus (Cluster IV). Cluster I was associated with increased risk of URI and sinusitis (RR = 1.18, p = 0.046; RR = 1.25, p = 0.009, respectively) in the population studied.
CONCLUSION
In a pediatric population, URI and sinusitis associate with the presence of Moraxella-dominated NP microbiota.
背景
已描述了儿科人群中不同的细菌上呼吸道微生物群结构，且其与呼吸道病毒感染风险以及哮喘加重相关。我们推测儿科人群中存在不同的鼻咽（NP）微生物群结构，其与环境暴露相关，并可改变儿童急性鼻窦炎或上呼吸道感染（URI）的风险。
方法
对58名4至7岁儿童在一年时间内纵向收集的鼻咽拭子（n = 354）的细菌16S rRNA谱进行分析，并与环境变量、URI和鼻窦炎结局相关联。
结果
鼻咽微生物群组成的差异与临床结局、参与者特征和环境暴露显著相关，包括优势细菌属、季节、日托出勤情况和烟草暴露。四种不同的鼻咽微生物群结构（聚类I-IV）明显不同，且在URI和鼻窦炎结局方面存在差异。这些聚类的特征分别为以莫拉克斯氏菌为主且基础分类群稀少（聚类I）、由非优势的多样化微生物群组成（聚类II）、以差异球菌/棒状杆菌为主（聚类III）或以嗜血杆菌为主（聚类IV）。在所研究的人群中，聚类I与URI和鼻窦炎风险增加相关（相对风险分别为1.18，p = 0.046；1.25，p = 0.009）。
结论
在儿科人群中，URI和鼻窦炎与以莫拉克斯氏菌为主的NP微生物群的存在相关。
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