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[2019 - 2020年马鞍山市公共场所淋浴水中的分子特征]

[Molecular characteristics of in shower water of public places in Ma'anshan city from 2019 to 2020].

作者信息

Wang L, Luo Y, Yang C, Li Y Y, Chen J, Wang J Q, Chen D L

机构信息

Center for Disease Control and Prevention of Ma'anshan 243000, China.

Health and Family Planning Supervision and Law Enforcement Bureau Ma'anshan 243000, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2021 Dec 6;55(12):1399-1403. doi: 10.3760/cma.j.cn112150-20210330-00313.

Abstract

To understand the epidemiological and pathogenic characteristics of in shower water of public places in Ma'anshan City from 2019 to 2020, and to provide scientific basis for further prevention and control of legionellosis. From 2019 to 2020, according to population density distribution and business scale of bathing places in the main urban area of Ma'anshan City (Huashan District and Yushan District), 8 public bathing places (including 3 large, 3 medium and 2 small) were selected to collect 308 shower water and water storage pool water samples (294 shower water samples and 14 water storage pool water samples). After the collected water samples were treated, cultured, isolated and identified, the type characteristics of were analyzed. were detected in 120 water samples among 308 shower water and water storage pool water samples, with an overall positive rate of 39.0% (120/308). A total of 154 strains were detected, including 10 different serotypes, predominated by serotype 1 (LP1) and serotype 3 (LP3), accounting for 40.9% (63/154) and 22.7% (35/154). Among 154 strains of , 23 strains of were positive for all 14 virulence genes, accounting for 14.9% (23/154), including 19 serotype 1 (LP1) and 4 serotype 8 (LP8). Pulsed field gel electrophoresis (PFGE) cluster analysis of 154 strains showed 60 different patterns. was seriously polluted in the shower water environment of public places in Ma anshan City. The serotypes are widely distributed and the virulence of the strains is strong. The results of molecular typing show that these strains have genetic diversity.

摘要

为了解2019年至2020年马鞍山市公共场所淋浴水中军团菌的流行病学及致病特征,为进一步防控军团菌病提供科学依据。2019年至2020年,根据马鞍山市主城区(花山区和雨山区)洗浴场所的人口密度分布及经营规模,选取8家公共洗浴场所(其中大型3家、中型3家、小型2家),采集308份淋浴水及储水池水样(淋浴水样294份、储水池水样14份)。对采集的水样进行处理、培养、分离和鉴定后,分析军团菌的型别特征。在308份淋浴水及储水池水样中,120份水样检测到军团菌,总体阳性率为39.0%(120/308)。共检测到154株军团菌菌株,包括10种不同血清型,以血清型1(LP1)和血清型3(LP3)为主,分别占40.9%(63/154)和22.7%(35/154)。在154株军团菌中,23株14个毒力基因均为阳性,占14.9%(23/154),其中血清型1(LP1)19株、血清型8(LP8)4株。对154株军团菌进行脉冲场凝胶电泳(PFGE)聚类分析,显示出60种不同图谱。马鞍山市公共场所淋浴水环境中军团菌污染严重。血清型分布广泛,菌株毒力较强。分子分型结果表明这些菌株具有遗传多样性。

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