Wang P Z, Projan S J, Leason K R, Novick R P
J Bacteriol. 1987 Jul;169(7):3082-7. doi: 10.1128/jb.169.7.3082-3087.1987.
A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide N-terminal extensions of 13 and 162 amino acids, and in both cases, the fusion protein was processed and the mature active enzyme was secreted; thus, the expression of a particular upstream gene can be analyzed by monitoring the beta-lactamase activity.
通过使用分泌蛋白葡萄球菌β-内酰胺酶作为指示物，开发了一种新型的翻译融合系统。β-内酰胺酶结构基因经过修饰，提供了13个和162个氨基酸的N端延伸，在这两种情况下，融合蛋白都经过加工，成熟的活性酶被分泌出来；因此，可以通过监测β-内酰胺酶活性来分析特定上游基因的表达。
