Nordan R P, Pumphrey J G, Rudikoff S
J Immunol. 1987 Aug 1;139(3):813-7.
Plasmacytoma growth factor (PCT-GF), a putative macrophage-derived lymphokine essential for the in vitro viability and proliferation of early generation plasmacytomas, was purified from conditioned medium of the murine macrophage cell line P388D1. The purification of PCT-GF was accomplished by a batch concentration on trimethylsilyl-controlled pore glass beads, followed by: gel filtration chromatography; hydrophobic interaction HPLC; and reverse-phase HPLC. SDS-PAGE analysis of the purified PCT-GF revealed a single band of Mr 23,000. The amino terminal sequence of PCT-GF was established as NH2-Pro-Thr-Ser-Gln-Val-Arg-Arg-Gly-Asp-Phe-Thr-Glu-Asp-Thr-Thr-Pro-Asn- Arg-Pro-Val-Tyr-Thr. No significant homology was found between this sequence and proteins in the National Biomedical Research Foundation database, suggesting that PCT-GF is a new lymphokine unrelated to previously described growth and differentiation factors.
浆细胞瘤生长因子（PCT - GF）是一种假定的巨噬细胞衍生的淋巴因子，对早期浆细胞瘤的体外存活和增殖至关重要，它是从鼠巨噬细胞系P388D1的条件培养基中纯化得到的。PCT - GF的纯化过程包括在三甲基硅烷基控制孔径玻璃珠上进行批量浓缩，随后依次进行：凝胶过滤色谱法、疏水相互作用高效液相色谱法和反相高效液相色谱法。对纯化后的PCT - GF进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析，显示出一条分子量为23,000的单一蛋白带。PCT - GF的氨基末端序列确定为NH2 - Pro - Thr - Ser - Gln - Val - Arg - Arg - Gly - Asp - Phe - Thr - Glu - Asp - Thr - Thr - Pro - Asn - Arg - Pro - Val - Tyr - Thr。在该序列与国家生物医学研究基金会数据库中的蛋白质之间未发现显著同源性，这表明PCT - GF是一种与先前描述的生长和分化因子无关的新型淋巴因子。
