Krenová D, Otová B, Kren V
Neoplasma. 1987;34(2):139-49.
LEW rat lymphatic leukemia/lymphoma was antigenically phenotyped by means of W3/13, OX7, P4/16 and F 17-23-2 MoAbs. T-cell lineage related markers were proven to be expressed by leukemia cells. AAS prepared in congenic rat strains have shown the following pattern: alpha RT1 (MHC) AAS directed against RT1 antigenic specificities both "public" and "private" gave positive reactions with 100% of leukemia cells, all cross-reacting AAS directed against "public" specificities only, reacted positively too with 17-100% of leukemia cells and no alien specificities have been detected when LEW antisera were tested. The expression of RT5 differentiation antigen was proved on leukemia cells by means of alpha RT5 congenic AAS. T-cell differentiation antigen RT6 was also detected by means of alpha RT6 AAS with closely similar specificity as MoAb P4/16 which also positively reacted with KPH-Lw-I cells. Leukemia T-cell origin is also supported by the absence of class II antigens (F 17-23-2 MoAb) and SIg receptors. A presence of leukemia/lymphoma associated antigen was indicated by AAS absorption analysis.
采用W3/13、OX7、P4/16和F 17-23-2单克隆抗体对LEW大鼠淋巴白血病/淋巴瘤进行抗原表型分析。已证实白血病细胞表达与T细胞系相关的标志物。在同源大鼠品系中制备的抗血清呈现出以下模式：针对RT1抗原“公共”和“私有”特异性的α RT1（MHC）抗血清与100%的白血病细胞呈阳性反应，所有仅针对“公共”特异性的交叉反应抗血清也与17%-100%的白血病细胞呈阳性反应，并且在检测LEW抗血清时未检测到外来特异性。通过α RT5同源抗血清证实白血病细胞上存在RT5分化抗原。还通过α RT6抗血清检测到T细胞分化抗原RT6，其特异性与单克隆抗体P4/16非常相似，P4/16也与KPH-Lw-I细胞呈阳性反应。白血病T细胞起源也得到了II类抗原（F 17-23-2单克隆抗体）和SIg受体缺失的支持。抗血清吸收分析表明存在白血病/淋巴瘤相关抗原。
