Soler-Garzón Alvaro, McClean Phillip E, Miklas Phillip N
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA, United States.
Department of Plant Sciences, North Dakota State University, Fargo, ND, United States.
Front Plant Sci. 2021 Dec 13;12:769247. doi: 10.3389/fpls.2021.769247. eCollection 2021.
(BCMV) is a major disease in common bean ( L.). Host plant resistance is the most effective strategy to minimize crop damage against BCMV and the related (BCMNV). To facilitate breeding for resistance, we sought to identify candidate genes and develop markers for the gene and the unknown gene with which it interacts. Genome-wide association study (GWAS) of the Durango Diversity Panel (DDP) identified a peak region for on chromosome Pv11. Haplotype mapping narrowed the genomic interval and identified Phvul.011G092700, a vacuolar protein-sorting 4 (Vps4) AAA+ ATPase endosomal sorting complexes required for transport (ESCRT) protein, as the candidate gene. The race Durango Phvul.011G092700 gene model, , contains a 10-kb deletion, while the race Mesoamerican consists of a single nucleotide polymorphism (SNP) deletion. Each mutation introduces a premature stop codon, and they exhibit the same interaction with the pathogroups (PGs) tested. Phvul.005G125100, another Vps4 AAA+ ATPase ESCRT protein, was identified as the candidate gene for the new recessive gene, and the recessive allele is likely an amino acid substitution in the microtubule interacting and transport (MIT) domain. The two Vps4 AAA+ ATPase ESCRT proteins exhibit high similarity to the Cucsa.385040 candidate gene associated with recessive resistance to in cucumber. alone has no resistance effect but, when combined with , provides resistance to BCMV (except PG-V) but not BCMNV, and, when combined with , provides resistance to BCMV (except BCMV PG-VII) and BCMNV. So instead of different resistance alleles (i.e., and ), there is only with a differential reaction based on whether it is combined with or , which are tightly linked in repulsion. The new tools and enhanced understanding of this host-virus pathogen interaction will facilitate breeding common beans for resistance to BCMV and BCMNV.
菜豆普通花叶病毒（BCMV）是菜豆（Phaseolus vulgaris L.）的一种主要病害。寄主植物抗性是将作物因BCMV及相关的菜豆普通花叶坏死病毒（BCMNV）造成的损害降至最低的最有效策略。为促进抗性育种，我们试图鉴定候选基因，并开发针对抗性基因及其相互作用的未知基因的标记。对杜兰戈多样性群体（DDP）进行全基因组关联研究（GWAS），在Pv11染色体上确定了一个与抗性相关的峰值区域。单倍型图谱缩小了抗性基因的基因组区间，并确定液泡蛋白分选4（Vps4）AAA + ATP酶内体分选转运复合体（ESCRT）蛋白Phvul.011G092700为抗性候选基因。杜兰戈小种Phvul.011G092700基因模型（phy011G092700）包含一个10 kb缺失，而中美洲小种由一个单核苷酸多态性（SNP）缺失组成。每个突变都引入了一个提前终止密码子，并且它们与所测试的致病型（PGs）表现出相同的相互作用。另一个Vps4 AAA + ATP酶ESCRT蛋白Phvul.005G125100被确定为新的隐性抗性基因的候选基因，隐性等位基因可能是微管相互作用与转运（MIT）结构域中的一个氨基酸替换。这两个Vps4 AAA + ATP酶ESCRT蛋白与黄瓜中与对西瓜花叶病毒隐性抗性相关的Cucsa.385040候选基因高度相似。单独的phy005G125100没有抗性作用，但与phy011G092700组合时，对BCMV（PG - V除外）具有抗性，但对BCMNV没有抗性，与phy011G092700组合时，对BCMV（BCMV PG - VII除外）和BCMNV具有抗性。因此，不是存在不同的抗性等位基因（即phy011G092700和phy005G125100），而是只有phy005G125100，其根据是否与phy011G092700组合而产生不同反应，phy011G092700和phy005G125100紧密连锁且相互排斥。这些新工具以及对这种寄主 - 病毒病原体相互作用的深入理解将有助于菜豆抗BCMV和BCMNV的育种。
