Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innrain 80/82, Innsbruck, Tyrol, 6020, Austria.
Institute of Microbiology, University of Innsbruck, Technikerstraße 25, Innsbruck, Tyrol, 6020, Austria.
J Sep Sci. 2022 Mar;45(5):1031-1041. doi: 10.1002/jssc.202100869. Epub 2022 Jan 19.
As recently shown, some fungal pigments exhibit significant photoactivity turning them into promising agents for the photodynamic treatment of microbial infections or malignant diseases. In the present study, a separation strategy for fungal anthraquinones was developed based on centrifugal partition chromatography. A suitable method was explored employing a methanolic extract of the fruiting bodies of Cortinarius sanguineus (Agaricales, Basidiomycota). An excellent fractionation was achieved using a biphasic solvent system comprising chloroform/ethyl acetate/methanol/water/acetic acid (3:1:3:2:1, v/v/v/v/v) operating in ascending mode. Experiments on an analytical scale with extracts of closely related Cortinarius species exhibited broad applicability of the devised system. Up to six pigments could be purified directly from the crude extract. Preparative-scale fractionation of the methanol extracts of C. malicorius and C. sanguineus demonstrated that up-scaling was possible without compromising selectivity.
如最近所显示的那样，一些真菌色素表现出显著的光活性，使它们成为治疗微生物感染或恶性疾病的光动力疗法的有前途的药物。在本研究中，基于离心分配色谱法开发了一种真菌蒽醌的分离策略。探索了一种合适的方法，使用来自红缘红菇（伞菌目，担子菌门）子实体的甲醇提取物。使用包含氯仿/乙酸乙酯/甲醇/水/乙酸（3:1:3:2:1，v/v/v/v/v）的两相溶剂系统以升序模式操作，可实现出色的分级。在与相关的红菇属物种提取物进行的分析规模实验中，所设计的系统具有广泛的适用性。多达六种色素可以直接从粗提取物中纯化。对 C. malicorius 和 C. sanguineus 的甲醇提取物进行制备规模的分级分离表明，在不影响选择性的情况下可以进行放大。
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