Department of Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology ISAN Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand.
Neglected, Zoonosis and Vector Borne Disease Research Group, Khon Kaen University, Khon Kaen 40002, Thailand.
Asian Pac J Cancer Prev. 2021 Dec 1;22(12):3903-3912. doi: 10.31557/APJCP.2021.22.12.3903.
Opisthorchiasis which exerted by infection of Opisthorchis viverrini is strongly related to the incident of cholangiocarcinoma (CCA) in many Southeast Asian countries northeastern of Thailand. The O. viverrini infection is primarily caused by raw fish consumption, and repeated exposure to liver fluke. Meanwhile, acetaminophen is usually medicated to relieve pain in particularly people in northeast Thailand.
This study therefore aimed at investigating effects of acetaminophen on pathogenesis in hamsters for opisthorchiasis.
There were 4 groups of hamsters: i) uninfected hamster (N); ii) sole acetaminophen administration (N-Ac); iii) sole O. viverrini infection (OV); and iv) combination of O. viverrini infection and acetaminophen (OV-Ac) on pathology of hamsters for 1 month post infection. For analysis of histopathological changes through hematoxylin and eosin, Sirius red and immunohistostaining for Cytokeratin 19 (CK-19), Proliferating cell nuclear antigen (PCNA) and CA 19-9, serum's hamsters were used detected for liver function tests and tumor-related genes expression.
After 1 month under these treatments, the OV-Ac showed significantly higher CCA risk, including inflammatory cells were aggregations around bile duct, new bile duct and fibrosis in subcapsular hepatic tissues, than other treatments. These pathological parameters were positively correlated with immunohistochemical staining derived from CK-19, PCNA and CA 19-9. In addition, OV-Ac had significantly higher liver function tests (ALT).
Combined intake of liver fluke-contaminated raw fishes and acetaminophen rendered more severity of CCA than sole consumption of the contaminated raw fishes.
由华支睾吸虫感染引起的华支睾吸虫病与泰国东北部许多东南亚国家的胆管癌（CCA）的发生密切相关。华支睾吸虫感染主要是由生食鱼感染引起的，反复接触肝吸虫。同时，在泰国东北部，人们通常会服用对乙酰氨基酚来缓解疼痛。
本研究旨在研究对乙酰氨基酚对肝吸虫病仓鼠发病机制的影响。
有 4 组仓鼠：i）未感染仓鼠（N）；ii）仅给予对乙酰氨基酚（N-Ac）；iii）仅感染华支睾吸虫（OV）；iv）感染华支睾吸虫和对乙酰氨基酚（OV-Ac）组合在感染后 1 个月对仓鼠进行病理学分析。通过苏木精和伊红、天狼猩红和细胞角蛋白 19（CK-19）、增殖细胞核抗原（PCNA）和 CA 19-9 的免疫组织化学染色分析组织病理学变化，血清中的仓鼠用于检测肝功能试验和肿瘤相关基因的表达。
经过 1 个月的这些治疗后，OV-Ac 显示出明显更高的 CCA 风险，包括胆管周围炎性细胞聚集、新胆管和包膜下肝组织纤维化，比其他治疗更为严重。这些病理参数与 CK-19、PCNA 和 CA 19-9 的免疫组织化学染色呈正相关。此外，OV-Ac 的肝功能试验（ALT）明显更高。
食用受肝吸虫污染的生鱼和同时摄入对乙酰氨基酚会导致 CCA 的严重程度比单独食用受污染的生鱼更为严重。
