• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

印尼熊蜂蜂毒肽对 MCF-7 细胞的抗乳腺癌活性：一项体外研究。

Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia's Apis cerana: An In Vitro Study.

机构信息

Department of Chemical Engineering, Faculty of Engineering, University of Indonesia, Kampus UI Depok, Depok, West Java, 16424, Indonesia.

出版信息

Asian Pac J Cancer Prev. 2021 Dec 1;22(12):3913-3919. doi: 10.31557/APJCP.2021.22.12.3913.

DOI:10.31557/APJCP.2021.22.12.3913
PMID:34967571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9080376/
Abstract

OBJECTIVE

Breast cancer is the most common case of cancers. Apitheraphy has been traditionally used for abundance diseases. This study aims to evaluate and compare the anti-breast cancer activity of melittin from Indonesia's Apic cerana as a potential drug for treating breast cancer.

METHODS

Apis cerana bee venom (BV) was collected from a bee farm in Cikurutung, Bandung using an electrical venom device. The BV was then purified using the ÄKTA Start system and HiTrap™ SP HP cation exchange chromatography column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify melittin based on its molecular mass and lowry's protein assay to measure melittin concentration. Melittin cytotoxicity was measured with brine shrimp lethality test (BSLT), while MCF-7 breast cancer cells MTT assay was used to measure its anti-breast cancer activity, based on inhinition rate.

RESULTS

95.432 μg/mL melittin is purified from 62.8 mg/L BV, using  cation exchange chromatography. Melittin in vitro analysis with MCF-7 MTT assay is used to determine anti-breast cancer activity in dose dependent manner. Furthermore, melttin BSLT result showed a LC50 16.67675 μg/mL. Therefore, the MTT assay  was conducted in 5, 10 and 15 μg/mL with MCF-7 inhibition values of 0.768 ± 0.014, 3.303 ± 0.011, and 35.714 ± 0.009 %, respectively.

CONCLUSION

Indonesia's Apis cerana has the potential to be used as a therapeutic peptide for breast cancer treatment.

摘要

目的

乳腺癌是最常见的癌症病例。蜂疗传统上被用于治疗多种疾病。本研究旨在评估和比较印度尼西亚中华蜜蜂（Apis cerana）来源的蜂毒素（melittin）作为治疗乳腺癌的潜在药物的抗乳腺癌活性。

方法

使用电毒液设备从万隆 Cikurutung 的一个蜂场采集中华蜜蜂蜂毒（BV）。然后使用 ÄKTA Start 系统和 HiTrap™ SP HP 阳离子交换层析柱对 BV 进行纯化。基于其分子量，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）鉴定蜂毒素，并使用 Lowry 氏蛋白测定法测量蜂毒素浓度。使用盐水虾致死试验（BSLT）测量蜂毒素细胞毒性，而 MCF-7 乳腺癌细胞 MTT 试验则用于根据抑制率测量其抗乳腺癌活性。

结果

使用阳离子交换层析从 62.8mg/L BV 中纯化出 95.432μg/mL 的蜂毒素。在 MCF-7 MTT 试验中对蜂毒素进行体外分析，以剂量依赖的方式测定抗乳腺癌活性。此外，蜂毒素 BSLT 结果显示 LC50 为 16.67675μg/mL。因此，在 MCF-7 抑制值为 0.768±0.014、3.303±0.011 和 35.714±0.009%的情况下，在 5、10 和 15μg/mL 浓度下进行 MTT 试验。

结论

印度尼西亚中华蜜蜂具有作为治疗乳腺癌的治疗性肽的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/01a61454d0c2/APJCP-22-3913-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/e7f38c440ee6/APJCP-22-3913-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/d4840bc2f965/APJCP-22-3913-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/01a61454d0c2/APJCP-22-3913-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/e7f38c440ee6/APJCP-22-3913-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/d4840bc2f965/APJCP-22-3913-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c73/9080376/01a61454d0c2/APJCP-22-3913-g003.jpg

相似文献

1
Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia's Apis cerana: An In Vitro Study.印尼熊蜂蜂毒肽对 MCF-7 细胞的抗乳腺癌活性：一项体外研究。
Asian Pac J Cancer Prev. 2021 Dec 1;22(12):3913-3919. doi: 10.31557/APJCP.2021.22.12.3913.
2
Functional characterization of naturally occurring melittin peptide isoforms in two honey bee species, Apis mellifera and Apis cerana.两种蜜蜂（西方蜜蜂和中华蜜蜂）中天然存在的蜂毒肽肽异构体的功能表征。
Peptides. 2014 Mar;53:185-93. doi: 10.1016/j.peptides.2014.01.026. Epub 2014 Feb 8.
3
Anti-metastatic Effects of Bee Venom and Melittin in Breast Cancer Cells by Upregulation of BRMS1 and DRG1 Genes.蜂毒和蜂肽通过上调 BRMS1 和 DRG1 基因对乳腺癌细胞的抗转移作用。
Chem Biol Drug Des. 2024 Oct;104(4):e14637. doi: 10.1111/cbdd.14637.
4
Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells.蜂毒肽通过抑制乳腺癌细胞中的PI3K/Akt/mTOR信号通路来抑制表皮生长因子诱导的细胞迁移和侵袭。
Food Chem Toxicol. 2014 Jun;68:218-25. doi: 10.1016/j.fct.2014.03.022. Epub 2014 Mar 25.
5
Antioxidant activity and irritation property of venoms from Apis species.蜜蜂属蜂种毒液的抗氧化活性与刺激性
Toxicon. 2018 Apr;145:32-39. doi: 10.1016/j.toxicon.2018.02.049. Epub 2018 Mar 2.
6
Bee Venom Components as Therapeutic Tools against Prostate Cancer.蜂毒液成分作为治疗前列腺癌的工具。
Toxins (Basel). 2021 May 7;13(5):337. doi: 10.3390/toxins13050337.
7
Melittin-A Natural Peptide from Bee Venom Which Induces Apoptosis in Human Leukaemia Cells.蜂毒肽——一种来自蜜蜂毒液的天然肽，可诱导人白血病细胞凋亡。
Biomolecules. 2020 Feb 6;10(2):247. doi: 10.3390/biom10020247.
8
Bee venom suppresses PMA-mediated MMP-9 gene activation via JNK/p38 and NF-kappaB-dependent mechanisms.蜂毒通过 JNK/p38 和 NF-κB 依赖的机制抑制 PMA 介导的 MMP-9 基因激活。
J Ethnopharmacol. 2010 Feb 17;127(3):662-8. doi: 10.1016/j.jep.2009.12.007. Epub 2009 Dec 5.
9
The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer.新型蜜蜂和独居蜂蜂毒变体在炎症和癌症方面的药理学潜力。
Toxins (Basel). 2022 Nov 22;14(12):818. doi: 10.3390/toxins14120818.
10
Honey Bee Venom (Apis mellifera) Contains Anticoagulation Factors and Increases the Blood-clotting Time.蜜蜂毒液（意大利蜜蜂）含有抗凝因子并延长凝血时间。
J Pharmacopuncture. 2015 Dec;18(4):7-11. doi: 10.3831/KPI.2015.18.031.

引用本文的文献

1
Structural insights and predictive modelling of a novel anti-HER2 scFv and Leptulipin: a newly designed immunotoxin protein for HER2 positive cancers.一种新型抗HER2单链抗体片段和瘦素脂肽的结构见解与预测模型：一种新设计的用于HER2阳性癌症的免疫毒素蛋白。
Toxicol Res (Camb). 2025 May 24;14(3):tfaf060. doi: 10.1093/toxres/tfaf060. eCollection 2025 Jun.
2
Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Development and Therapeutical Efficacy and Safety Evaluation.载蜂毒的非离子表面活性剂囊泡作为癌症治疗的创新平台：研发、治疗效果及安全性评估
Pharmaceuticals (Basel). 2024 Apr 29;17(5):572. doi: 10.3390/ph17050572.
3

本文引用的文献

1
Bee Venom Components as Therapeutic Tools against Prostate Cancer.蜂毒液成分作为治疗前列腺癌的工具。
Toxins (Basel). 2021 May 7;13(5):337. doi: 10.3390/toxins13050337.
2
Cancer statistics for the year 2020: An overview.2020年癌症统计数据概述。
Int J Cancer. 2021 Apr 5. doi: 10.1002/ijc.33588.
3
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.《全球癌症统计数据 2020：全球 185 个国家和地区 36 种癌症的发病率和死亡率估计》。
Bee Venom: Composition and Anticancer Properties.
蜂毒：组成与抗癌特性。
Toxins (Basel). 2024 Feb 29;16(3):117. doi: 10.3390/toxins16030117.
4
Melitoxin Inhibits Proliferation, Metastasis, and Invasion of Glioma U251 Cells by Down-regulating F2RL1.蜂毒素通过下调F2RL1抑制胶质瘤U251细胞的增殖、转移和侵袭。
Appl Biochem Biotechnol. 2024 Sep;196(9):6234-6252. doi: 10.1007/s12010-023-04841-y. Epub 2024 Jan 22.
5
Melittin: a possible regulator of cancer proliferation in preclinical cell culture and animal models.蜂毒素：一种可能在临床前细胞培养和动物模型中调节癌症增殖的物质。
J Cancer Res Clin Oncol. 2023 Dec;149(19):17709-17726. doi: 10.1007/s00432-023-05458-8. Epub 2023 Nov 3.
6
Can Bee Venom Be Used as Anticancer Agent in Modern Medicine?蜂毒在现代医学中能否用作抗癌剂？
Cancers (Basel). 2023 Jul 21;15(14):3714. doi: 10.3390/cancers15143714.
7
Delivery of Melittin as a Lytic Agent via Graphene Nanoparticles as Carriers to Breast Cancer Cells.通过石墨烯纳米颗粒作为载体将蜂毒素作为一种溶解剂递送至乳腺癌细胞。
J Funct Biomater. 2022 Dec 7;13(4):278. doi: 10.3390/jfb13040278.
8
Pharmacological effects and mechanisms of bee venom and its main components: Recent progress and perspective.蜂毒及其主要成分的药理作用和机制：最新进展与展望
Front Pharmacol. 2022 Sep 27;13:1001553. doi: 10.3389/fphar.2022.1001553. eCollection 2022.
CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.
4
In-depth characterization of antibacterial activity of melittin against Staphylococcus aureus and use in a model of non-surgical MRSA-infected skin wounds.深入研究蜂毒素对金黄色葡萄球菌的抗菌活性及其在非手术耐甲氧西林金黄色葡萄球菌感染皮肤创面模型中的应用。
Eur J Pharm Sci. 2021 Jan 1;156:105592. doi: 10.1016/j.ejps.2020.105592. Epub 2020 Oct 10.
5
Clinical Applications of Bee Venom Acupoint Injection.蜂针穴位注射的临床应用。
Toxins (Basel). 2020 Sep 27;12(10):618. doi: 10.3390/toxins12100618.
6
Honeybee venom and melittin suppress growth factor receptor activation in HER2-enriched and triple-negative breast cancer.蜜蜂毒液和蜂毒肽可抑制HER2富集型和三阴性乳腺癌中生长因子受体的激活。
NPJ Precis Oncol. 2020 Sep 1;4:24. doi: 10.1038/s41698-020-00129-0. eCollection 2020.
7
Anti-fungal properties and mechanisms of melittin.蜂毒素的抗真菌特性和作用机制。
Appl Microbiol Biotechnol. 2020 Aug;104(15):6513-6526. doi: 10.1007/s00253-020-10701-0. Epub 2020 Jun 4.
8
Apamin from bee venom suppresses inflammation in a murine model of gouty arthritis.蜂毒中的蜂毒肽可抑制痛风性关节炎小鼠模型的炎症反应。
J Ethnopharmacol. 2020 Jul 15;257:112860. doi: 10.1016/j.jep.2020.112860. Epub 2020 Apr 11.
9
Melittin-A Natural Peptide from Bee Venom Which Induces Apoptosis in Human Leukaemia Cells.蜂毒肽——一种来自蜜蜂毒液的天然肽，可诱导人白血病细胞凋亡。
Biomolecules. 2020 Feb 6;10(2):247. doi: 10.3390/biom10020247.
10
Anti-tumor effects of an antagonistic mAb against the ASCT2 amino acid transporter on KRAS-mutated human colorectal cancer cells.针对 ASCT2 氨基酸转运蛋白的拮抗单抗对 KRAS 突变型人结直肠癌细胞的抗肿瘤作用。
Cancer Med. 2020 Jan;9(1):302-312. doi: 10.1002/cam4.2689. Epub 2019 Nov 10.