Department of Chemical Engineering, Faculty of Engineering, University of Indonesia, Kampus UI Depok, Depok, West Java, 16424, Indonesia.
Asian Pac J Cancer Prev. 2021 Dec 1;22(12):3913-3919. doi: 10.31557/APJCP.2021.22.12.3913.
Breast cancer is the most common case of cancers. Apitheraphy has been traditionally used for abundance diseases. This study aims to evaluate and compare the anti-breast cancer activity of melittin from Indonesia's Apic cerana as a potential drug for treating breast cancer.
Apis cerana bee venom (BV) was collected from a bee farm in Cikurutung, Bandung using an electrical venom device. The BV was then purified using the ÄKTA Start system and HiTrap™ SP HP cation exchange chromatography column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify melittin based on its molecular mass and lowry's protein assay to measure melittin concentration. Melittin cytotoxicity was measured with brine shrimp lethality test (BSLT), while MCF-7 breast cancer cells MTT assay was used to measure its anti-breast cancer activity, based on inhinition rate.
95.432 μg/mL melittin is purified from 62.8 mg/L BV, using cation exchange chromatography. Melittin in vitro analysis with MCF-7 MTT assay is used to determine anti-breast cancer activity in dose dependent manner. Furthermore, melttin BSLT result showed a LC50 16.67675 μg/mL. Therefore, the MTT assay was conducted in 5, 10 and 15 μg/mL with MCF-7 inhibition values of 0.768 ± 0.014, 3.303 ± 0.011, and 35.714 ± 0.009 %, respectively.
Indonesia's Apis cerana has the potential to be used as a therapeutic peptide for breast cancer treatment.
乳腺癌是最常见的癌症病例。蜂疗传统上被用于治疗多种疾病。本研究旨在评估和比较印度尼西亚中华蜜蜂（Apis cerana）来源的蜂毒素（melittin）作为治疗乳腺癌的潜在药物的抗乳腺癌活性。
使用电毒液设备从万隆 Cikurutung 的一个蜂场采集中华蜜蜂蜂毒（BV）。然后使用 ÄKTA Start 系统和 HiTrap™ SP HP 阳离子交换层析柱对 BV 进行纯化。基于其分子量，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）鉴定蜂毒素，并使用 Lowry 氏蛋白测定法测量蜂毒素浓度。使用盐水虾致死试验（BSLT）测量蜂毒素细胞毒性，而 MCF-7 乳腺癌细胞 MTT 试验则用于根据抑制率测量其抗乳腺癌活性。
使用阳离子交换层析从 62.8mg/L BV 中纯化出 95.432μg/mL 的蜂毒素。在 MCF-7 MTT 试验中对蜂毒素进行体外分析，以剂量依赖的方式测定抗乳腺癌活性。此外，蜂毒素 BSLT 结果显示 LC50 为 16.67675μg/mL。因此，在 MCF-7 抑制值为 0.768±0.014、3.303±0.011 和 35.714±0.009%的情况下，在 5、10 和 15μg/mL 浓度下进行 MTT 试验。
印度尼西亚中华蜜蜂具有作为治疗乳腺癌的治疗性肽的潜力。
Zotero 插件
