Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, South Korea.
Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, 35015, South Korea; Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015, South Korea.
J Ethnopharmacol. 2020 Jul 15;257:112860. doi: 10.1016/j.jep.2020.112860. Epub 2020 Apr 11.
Bee venom (BV) has been used for the treatment of inflammatory diseases, such as rheumatoid arthritis, and for the relief of pain in traditional oriental medicine.
The aim of this study was to determine the anti-inflammatory effect of BV on monosodium urate (MSU)-induced gouty arthritis in a mouse model.
To develop a mouse model of acute gouty arthritis, 4 mg 50 μL of MSU crystal suspension was injected intradermally into the right paw. After MSU crystal injection, we evaluated inflammatory cytokine production in mice of the BV-treated (0.5 and 1 mg kg body weight) and apamin (APM)-treated (0.5 and 1 mg kg body weight) groups. The positive control group was administered a colchicine (1 mg kg body weight) injection with MSU crystals.
BV and APM treatment suppressed inflammatory paw edema in MSU-administered mice. It also exerted anti-inflammatory effects in mice with gouty arthritis by inhibiting proinflammatory cytokine production and inflammasome formation. Interestingly, MSU crystal formation was decreased by BV and APM treatment.
These results suggest that the APM from BV might be useful for the treatment of gouty arthritis due to its anti-inflammatory activities.
蜂毒（BV）已被用于治疗炎症性疾病，如类风湿性关节炎，并在传统东方医学中用于缓解疼痛。
本研究旨在确定 BV 对尿酸单钠（MSU）诱导的痛风性关节炎在小鼠模型中的抗炎作用。
为了建立急性痛风性关节炎的小鼠模型，将 4mg 50μL 的 MSU 晶体混悬液皮内注射到右爪中。在 MSU 晶体注射后，我们评估了 BV 处理（0.5 和 1mg/kg 体重）和蜂毒肽（APM）处理（0.5 和 1mg/kg 体重）组小鼠的炎性细胞因子产生情况。阳性对照组用秋水仙碱（1mg/kg 体重）注射 MSU 晶体。
BV 和 APM 治疗抑制了 MSU 给药小鼠的炎症性爪肿胀。它还通过抑制促炎细胞因子的产生和炎性小体的形成，对痛风性关节炎小鼠发挥抗炎作用。有趣的是，BV 和 APM 治疗降低了 MSU 晶体的形成。
这些结果表明，BV 中的 APM 可能因其抗炎活性而有助于痛风性关节炎的治疗。
