Linder E, Lehto V P
Scand J Immunol. 1978 Mar;7(3):239-44. doi: 10.1111/j.1365-3083.1978.tb00450.x.
Sera from patients with chronic inflammatory disease were tested for the presence of connective tissue antibodies. Fetal human skin was used as a substrate in an indirect immunofluorescence assay. Histological staining for connective tissue fibres, antibodies to connective tissue components and immunoelectron microscopy were used to identify the antigenic structures. Some antigenic fibrils were associated with elastin. By immunoelectron microscopy antigenic extracellular microfibrillar structures were identified. Antibodies against these microfibrils were detected in higher titres only in chronic inflammation (14%). Our results suggest that antibodies against connective tissues microfibrils may occur in human sera.
对慢性炎症性疾病患者的血清进行结缔组织抗体检测。在间接免疫荧光试验中，使用人胎儿皮肤作为底物。采用结缔组织纤维的组织学染色、针对结缔组织成分的抗体检测及免疫电子显微镜技术来鉴定抗原结构。一些抗原性原纤维与弹性蛋白相关。通过免疫电子显微镜鉴定出抗原性细胞外微纤维结构。仅在慢性炎症患者中（14%）检测到针对这些微纤维的抗体具有更高的滴度。我们的结果表明，针对结缔组织微纤维的抗体可能存在于人类血清中。
