Golds E E, Poole A R
Cell Immunol. 1984 Jun;86(1):190-205. doi: 10.1016/0008-8749(84)90372-1.
Peripheral blood mononuclear cells from patients with rheumatoid arthritis (n = 27), systemic lupus erythematosus (n = 24), juvenile rheumatoid arthritis (n = 30), osteoarthritis (n = 20), apparently healthy adults (n = 12), and nonarthritic children (n = 8) were exposed to several putative connective tissue antigens to determine if the monokine, mononuclear cell factor, was released. Release of this factor was detected by bioassay in which enhancement of collagenase production from human synovial cells or dermal fibroblasts was measured. The antigens, all of homologous tissue origin, included cyanogen bromide-derived peptides of type I, II, and III collagens, type I and II helical collagens, and cartilage proteoglycan. Of the subjects examined, 44% of the rheumatoid group, 42% of the systemic lupus group, 33% of the juvenile rheumatoid group but only 10% of the osteoarthritic group and 5% of the control group released monokine after exposure of peripheral blood mononuclear cells to at least one of these connective tissue antigens. Patients with rheumatoid arthritis most frequently responded to type II peptides (but not to type II helical collagen) although the frequencies of responses to type I peptides, type I helical collagen and proteoglycan were also elevated over levels observed in the control population. Positive responses in these patients typically occurred to only one antigen, were transient, often occurred close to the onset of arthritis, and appeared to be unrelated to disease activity. The profiles of responses in patients with juvenile rheumatoid arthritis and systemic lupus shared many features in common and were distinct from those of adult rheumatoid arthritis. Patients with systemic lupus or juvenile rheumatoid arthritis responded to all of the antigens tested. Positive responses often occurred simultaneously to several antigens. Responses to type II helical collagen were most common while sensitization to type II peptides was infrequently detected. Positive responses were transient, unrelated to overall disease activity, type of juvenile arthritis, or duration of disease in lupus patients. Stimulation of mononuclear cell factor release by connective tissue molecules and their degradation products may make an important contribution to the chronic inflammation commonly seen in these diseases.
将类风湿性关节炎患者（n = 27）、系统性红斑狼疮患者（n = 24）、青少年类风湿性关节炎患者（n = 30）、骨关节炎患者（n = 20）、表面健康的成年人（n = 12）以及非关节炎儿童（n = 8）的外周血单核细胞暴露于几种假定的结缔组织抗原中，以确定是否会释放单核因子（单核细胞因子）。通过生物测定法检测该因子的释放，其中测量人滑膜细胞或真皮成纤维细胞胶原酶产生的增强情况。所有抗原均来自同源组织，包括I型、II型和III型胶原的溴化氰衍生肽、I型和II型螺旋胶原以及软骨蛋白聚糖。在所检查的受试者中，类风湿组44%、系统性红斑狼疮组42%、青少年类风湿组33%，但骨关节炎组仅10%，对照组仅5%的外周血单核细胞在暴露于至少一种这些结缔组织抗原后释放单核因子。类风湿性关节炎患者对II型肽（但对II型螺旋胶原无反应）反应最为频繁，尽管对I型肽、I型螺旋胶原和蛋白聚糖的反应频率也高于对照组人群中观察到的水平。这些患者的阳性反应通常仅针对一种抗原，是短暂的，常发生在关节炎发作附近，且似乎与疾病活动无关。青少年类风湿性关节炎和系统性红斑狼疮患者的反应模式有许多共同特征，且与成人类风湿性关节炎不同。系统性红斑狼疮或青少年类风湿性关节炎患者对所有测试抗原均有反应。阳性反应常同时针对几种抗原。对II型螺旋胶原的反应最为常见，而对II型肽的致敏则很少检测到。阳性反应是短暂的，与总体疾病活动、青少年关节炎类型或狼疮患者的疾病持续时间无关。结缔组织分子及其降解产物对单核细胞因子释放的刺激可能对这些疾病中常见的慢性炎症起重要作用。
