EpiDiverse植物全基因组表观遗传关联研究（EWAS）流程

The EpiDiverse Plant Epigenome-Wide Association Studies (EWAS) Pipeline.

作者信息

Can Sultan Nilay, Nunn Adam, Galanti Dario, Langenberger David, Becker Claude, Volmer Katharina, Heer Katrin, Opgenoorth Lars, Fernandez-Pozo Noe, Rensing Stefan A

机构信息

Plant Cell Biology, Department of Biology, University of Marburg, 35043 Marburg, Germany.

ecSeq Bioinformatics GmbH, 04103 Leipzig, Germany.

出版信息

Epigenomes. 2021 May 4;5(2):12. doi: 10.3390/epigenomes5020012.

DOI:10.3390/epigenomes5020012

PMID:34968299

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8594691/

Abstract

Bisulfite sequencing is a widely used technique for determining DNA methylation and its relationship with epigenetics, genetics, and environmental parameters. Various techniques were implemented for epigenome-wide association studies (EWAS) to reveal meaningful associations; however, there are only very few plant studies available to date. Here, we developed the EpiDiverse EWAS pipeline and tested it using two plant datasets, from (Norway spruce) and (valley oak). Hence, we present an EWAS implementation tested for non-model plant species and describe its use.

摘要

亚硫酸氢盐测序是一种广泛用于确定DNA甲基化及其与表观遗传学、遗传学和环境参数关系的技术。为了揭示有意义的关联，人们采用了各种技术进行全表观基因组关联研究（EWAS）；然而，迄今为止，植物研究非常少。在这里，我们开发了EpiDiverse EWAS流程，并使用来自挪威云杉和山谷橡树的两个植物数据集对其进行了测试。因此，我们展示了一种针对非模式植物物种进行测试的EWAS实施方案，并描述了其用途。