Cho Yung-Chieh, Peng Pei-Wen, Ou Yu-Sin, Liu Chung-Ming, Huang Bai-Hung, Lan Wen-Chien, Kuo Hsin-Hui, Hsieh Chia-Chien, Chen Brian, Huang Mao-Suan, Nakano Hiroyuki
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
Materials (Basel). 2024 May 11;17(10):2276. doi: 10.3390/ma17102276.
The present study investigated the in vivo bone-forming efficacy of an innovative titanium (Ti) dental implant combined with a collagen sponge containing recombinant human bone morphogenetic protein-2 (BMP-2) in a pig model. Two different concentrations of BMP-2 (20 and 40 µg/mL) were incorporated into collagen sponges and placed at the bottom of Ti dental implants. The investigated implants were inserted into the edentulous ridge at the canine-premolar regions of Lanyu small-ear pigs, which were then euthanized at weeks 1, 2, 4, 8, and 12 post-implantation. Specimens containing the implants and surrounding bone tissue were collected for histological evaluation of their bone-to-implant contact (BIC) ratios and calculation of maximum torques using removal torque measurement. Analytical results showed that the control and BMP-2-loaded implants presented good implant stability and bone healing for all testing durations. After 1 week of healing, the BMP-2-loaded implants with a concentration of 20 µg/mL exhibited the highest BIC ratios, ranging from 58% to 76%, among all groups ( = 0.034). Additionally, they also possessed the highest removal torque values (50.1 ± 1.3 N-cm) throughout the 8-week healing period. The BMP-2-loaded implants not only displayed excellent in vivo biocompatibility but also presented superior osteoinductive performance. Therefore, these findings demonstrate that BMP-2 delivered through a collagen sponge can potentially enhance the early-stage osseointegration of Ti dental implants.
本研究在猪模型中探究了一种创新的钛（Ti）牙科种植体与含重组人骨形态发生蛋白-2（BMP-2）的胶原海绵联合使用时的体内成骨效果。将两种不同浓度的BMP-2（20和40μg/mL）掺入胶原海绵中，并置于Ti牙科种植体底部。将研究用种植体植入兰屿小耳猪犬前磨牙区域的无牙牙槽嵴，然后在植入后第1、2、4、8和12周实施安乐死。收集含有种植体及周围骨组织的标本，用于组织学评估骨与种植体接触（BIC）比率，并通过去除扭矩测量计算最大扭矩。分析结果表明，在所有测试时间段内，对照种植体和负载BMP-2的种植体均表现出良好的种植体稳定性和骨愈合情况。愈合1周后，浓度为20μg/mL的负载BMP-2的种植体在所有组中表现出最高的BIC比率，范围为58%至76%（ = 0.034）。此外，在整个8周愈合期内，它们还具有最高的去除扭矩值（50.1±1.3 N·cm）。负载BMP-2的种植体不仅在体内显示出优异的生物相容性，还表现出卓越的骨诱导性能。因此，这些研究结果表明，通过胶原海绵递送的BMP-2可能会增强Ti牙科种植体的早期骨整合。
