Lu Quanwei, Xiao Xianghui, Gong Juwu, Li Pengtao, Zhao Yan, Feng Jiajia, Peng Renhai, Shi Yuzhen, Yuan Youlu
School of Biotechnology and Food Engineering, Anyang Institute of Technology, Anyang, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
Front Plant Sci. 2021 Dec 14;12:796722. doi: 10.3389/fpls.2021.796722. eCollection 2021.
Fiber length is an important determinant of fiber quality, and it is a quantitative multi-genic trait. Identifying genes associated with fiber length is of great importance for efforts to improve fiber quality in the context of cotton breeding. Integrating transcriptomic information and details regarding candidate gene regions can aid in candidate gene identification. In the present study, the CCRI45 line and a chromosome segment substitution line (CSSL) with a significantly higher fiber length (MBI7747) were utilized to establish F and F populations. Using a high-density genetic map published previously, six quantitative trait loci (QTLs) associated with fiber length and two QTLs associated with fiber strength were identified on four chromosomes. Within these QTLs, , , , and were identified in two or three environments and confirmed by a meta-analysis. By integrating transcriptomic data from the two parental lines and through qPCR analyses, four genes associated with these QTLs including Cellulose synthase-like protein D3 (, for ), expansin-A1 (, for ), plasmodesmata callose-binding protein 3 (, for ), and Polygalacturonase (, for ) were identified as promising candidate genes associated with fiber length. Overall, these results offer a robust foundation for further studies regarding the molecular basis for fiber length and for efforts to improve cotton fiber quality.
纤维长度是纤维品质的一个重要决定因素，并且它是一个数量多基因性状。在棉花育种背景下，鉴定与纤维长度相关的基因对于提高纤维品质的努力非常重要。整合转录组信息和候选基因区域的详细信息有助于鉴定候选基因。在本研究中，利用中棉所45品系和一个纤维长度显著更长的染色体片段代换系（CSSL，MBI7747）构建了F和F群体。利用先前发表的高密度遗传图谱，在四条染色体上鉴定出了6个与纤维长度相关的数量性状位点（QTL）和2个与纤维强度相关的QTL。在这些QTL中，、、、和在两个或三个环境中被鉴定出来，并通过荟萃分析得到确认。通过整合两个亲本系的转录组数据并进行qPCR分析，鉴定出了4个与这些QTL相关的基因，包括类纤维素合酶蛋白D3（，对应）、扩展蛋白-A1（，对应）、胞间连丝胼胝质结合蛋白3（，对应）和多聚半乳糖醛酸酶（，对应），这些基因被认为是与纤维长度相关的有前景的候选基因。总体而言，这些结果为进一步研究纤维长度的分子基础以及提高棉花纤维品质的努力提供了坚实的基础。
