Liu Zhiwei, Chen Jiayi, Zhao Sijun, Pang Yimeng, Shen Xing, Lei Hongtao, Li Xiangmei
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
China Animal Health and Epidemiology Center, 369 Nanjing Rd, Si Fang Qu, Qingdao, 266032, China.
Mikrochim Acta. 2021 Dec 31;189(1):42. doi: 10.1007/s00604-021-05151-y.
Three kinds of immunochromatographic assays (ICAs) are proposed for the highly sensitive and rapid determination of tylosin (TYL) and tilmicosin (TIM) in eggs based on colloidal gold (CG), latex microsphere (LM), and time-resolved fluorescent microsphere (TRFM). Three types of ICAs could tolerate the egg matrix via simple sample pretreatment and demonstrated high sensitivity for TYL and TIM with cut-off values of 6/6/3 μg/kg and 14/14/6 μg/kg, respectively. Furthermore, in a single-blind parallel study 20 egg samples were analyzed by the three developed ICAs and confirmed by LC-MS/MS. The results showed good consistency, and there were no false positive and false negative results in our three ICAs. Consequently, the proposed three ICAs offered rapid, highly sensitive, reliable, and selectable testing platforms for screening veterinary medicine or other small molecule contaminants.
基于胶体金（CG）、乳胶微球（LM）和时间分辨荧光微球（TRFM），提出了三种免疫色谱分析法（ICA），用于高灵敏、快速地测定鸡蛋中的泰乐菌素（TYL）和替米考星（TIM）。通过简单的样品预处理，三种类型的ICA能够耐受鸡蛋基质，并且对TYL和TIM表现出高灵敏度，其截断值分别为6/6/3 μg/kg和14/14/6 μg/kg。此外，在一项单盲平行研究中，用三种开发的ICA对20个鸡蛋样品进行了分析，并通过液相色谱-串联质谱法（LC-MS/MS）进行了确认。结果显示出良好的一致性，并且我们的三种ICA均未出现假阳性和假阴性结果。因此，所提出的三种ICA为筛查兽药或其他小分子污染物提供了快速、高灵敏、可靠且可选择的检测平台。
