He Zixian, Liu Zhiwei, Xie Haihuan, Luo Pengjie, Li Xiangmei
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Wuzhou Institute for Food and Drug Control, Wuzhou 543099, China.
Foods. 2023 Jan 25;12(3):539. doi: 10.3390/foods12030539.
Herein, an ultrasensitive lateral flow immunoassay (LFIA), based on metal-organic framework-decorated polydopamine (PCN-224@PDA) was first established to detect multiple sulfonylureas (SUs) in functional foods. The PCN-224@PDA was synthesized using the one-pot hydrothermal method and covalently coupled with SUs antibodies, and the coupling rate was up to 91.8%. The detection limits of the developed PCN-224@PDA-LFIA for multiple SUs in functional teas and capsules were 0.22-3.72 μg/kg and 0.40-3.71 μg/kg, and quantification limits were 0.75-8.19 μg/kg and 1.03-9.08 μg/kg, respectively. The analytical sensitivity was 128-fold higher than that of similar methods reported so far. The recovery rates ranged from 83.8 to 119.0%, with coefficients of variation of 7.6-14.4%. The parallel analysis of 20 real samples by LC-MS/MS confirmed the reliability of the proposed method. Therefore, our work offers novel, ultrasensitive, and rapid technical support for on-site monitoring of SUs in functional foods.
在此，首次建立了一种基于金属有机框架修饰的聚多巴胺（PCN-224@PDA）的超灵敏侧向流动免疫分析（LFIA）方法，用于检测功能性食品中的多种磺酰脲类（SUs）。采用一锅水热法合成PCN-224@PDA，并将其与SUs抗体共价偶联，偶联率高达91.8%。所开发的PCN-224@PDA-LFIA对功能性茶和胶囊中多种SUs的检测限分别为0.22 - 3.72 μg/kg和0.40 - 3.71 μg/kg，定量限分别为0.75 - 8.19 μg/kg和1.03 - 9.08 μg/kg。分析灵敏度比迄今报道的类似方法高128倍。回收率在83.8%至119.0%之间，变异系数为7.6 - 14.4%。通过LC-MS/MS对20个实际样品进行平行分析，证实了该方法的可靠性。因此，我们的工作为功能性食品中SUs的现场监测提供了新颖、超灵敏且快速的技术支持。
