Department of Orthopedics, The Second Xiangya Hospital, Central South University, 410011, China; Orthopedic Biomedical Materials Engineering Laboratory of Hunan Province, Changsha 410011, China.
School of Materials Science and Engineering, Xiangtan University, Xiangtan, Hunan 411105, China.
Acta Biomater. 2022 Mar 15;141:454-465. doi: 10.1016/j.actbio.2021.12.032. Epub 2021 Dec 28.
Magnesium (Mg) and some of its alloys are considered promising biodegradable metallic biomaterials for bone implant applications. The osteogenesis effect of Mg alloys is widely reported; however, the underlying mechanisms are still not clear. In this study, pure Mg, Mg-3Zn, and Mg-2Zn-1Mn were prepared, and their degradation behavior, biocompatibility, and osteogenesis effect were systematically assessed both in vitro and in vivo. Primary rat bone marrow-derived mesenchymal stem cells (BMSCs) were used to evaluate the biocompatibility of the prepared Mg alloys, and a rat femur fracture model was used to assess the stimulating effect of these alloys on bone-tissue formation. Mg-2Zn-1Mn showed higher corrosion resistance and more stable degradation behavior than pure Mg and Mg-3Zn. Extracts of the three materials showed significant stimulating effects on osteogenic differentiation of BMSCs along with non-cytotoxicity. Implantation of Mg-2Zn-1Mn wires into the femur of rats demonstrated superior histocompatibility, stable degradation, and notable promotion of osteogenesis without systemic toxicity. Moreover, the results of both in vitro and in vivo assessments demonstrated that bone morphogenetic proteins and fibroblast growth factor receptors are involved in the stimulating effect of Mg alloys. STATEMENT OF SIGNIFICANCE: This work reports the degradation behavior, biocompatibility, and osteogenic effect of pure Mg and Mg-3Zn and Mg-2Zn-1Mn alloys in both in vitro and in vivo conditions. Mg-2Zn-1Mn showed higher corrosion resistance and more stable degradation behavior than pure Mg and Mg-3Zn. The extracts of the three materials showed a significant stimulating effect on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) along with non-cytotoxicity. Mg-2Zn-1Mn wires implanted into the femur of rats showed good histocompatibility, stable degradation, and notable promotion of osteogenesis without systemic toxicity. The results of the present study suggest that bone morphogenetic proteins (BMPs) and fibroblast growth factor receptors (FGFRs) are involved in the stimulating effect of Mg alloys on osteogenesis.
镁（Mg）及其一些合金被认为是有前途的可生物降解的金属生物材料，可用于骨植入应用。Mg 合金的成骨作用已被广泛报道，但潜在的机制仍不清楚。在这项研究中，制备了纯镁、Mg-3Zn 和 Mg-2Zn-1Mn，并在体外和体内系统评估了它们的降解行为、生物相容性和成骨作用。原代大鼠骨髓间充质干细胞（BMSCs）用于评估所制备的 Mg 合金的生物相容性，大鼠股骨骨折模型用于评估这些合金对骨组织形成的刺激作用。Mg-2Zn-1Mn 表现出比纯镁和 Mg-3Zn 更高的耐腐蚀性和更稳定的降解行为。三种材料的提取物对 BMSCs 的成骨分化具有显著的刺激作用，同时无细胞毒性。将 Mg-2Zn-1Mn 丝植入大鼠股骨中表现出良好的组织相容性、稳定的降解和明显的成骨促进作用，而无全身毒性。此外，体外和体内评估的结果表明，骨形态发生蛋白（BMPs）和成纤维细胞生长因子受体（FGFRs）参与了 Mg 合金的刺激作用。
本工作报告了纯镁和 Mg-3Zn 和 Mg-2Zn-1Mn 合金在体外和体内条件下的降解行为、生物相容性和成骨作用。Mg-2Zn-1Mn 比纯镁和 Mg-3Zn 具有更高的耐腐蚀性和更稳定的降解行为。三种材料的提取物对大鼠骨髓间充质干细胞（BMSCs）的成骨分化具有显著的刺激作用，同时无细胞毒性。植入大鼠股骨的 Mg-2Zn-1Mn 丝表现出良好的组织相容性、稳定的降解和明显的成骨促进作用，而无全身毒性。本研究结果表明，骨形态发生蛋白（BMPs）和成纤维细胞生长因子受体（FGFRs）参与了 Mg 合金对成骨的刺激作用。
