Pemberton R M, Cannon M J, Openshaw P J, Ball L A, Wertz G W, Askonas B A
J Gen Virol. 1987 Aug;68 ( Pt 8):2177-82. doi: 10.1099/0022-1317-68-8-2177.
We examined the specificity of BALB/c cytotoxic T (Tc) cells for respiratory syncytial virus (RSV) components, using recombinant vaccinia viruses (VV) coding for several individual RSV proteins. We found that immunization with the different VVs yielded the following Tc memory cell populations: high levels of RSV-specific Tc cells were induced with the fusion protein VV, but low levels were induced with VV coding for the RSV nucleoprotein. Tc cell recognition of attachment glycoprotein, part of the matrix molecule or 1A internal protein was poor. While high levels of fusion protein-specific Tc cells were induced by the fusion protein VV, they showed poor cross-reactivity between the A2 and 8/60 RSV strains compared with Tc cells primed by RSV infection.
我们使用编码几种呼吸道合胞病毒（RSV）单个蛋白的重组痘苗病毒（VV），检测了BALB/c细胞毒性T（Tc）细胞对RSV组分的特异性。我们发现，用不同的VV进行免疫产生了以下Tc记忆细胞群体：融合蛋白VV诱导产生高水平的RSV特异性Tc细胞，但编码RSV核蛋白的VV诱导产生的水平较低。Tc细胞对附着糖蛋白、基质分子的一部分或1A内部蛋白的识别较差。虽然融合蛋白VV诱导产生了高水平的融合蛋白特异性Tc细胞，但与由RSV感染引发的Tc细胞相比，它们在A2和8/60 RSV毒株之间显示出较差的交叉反应性。
