鸢尾素（FNDC5）在结直肠癌中的表达意义。
Significance of Irisin (FNDC5) Expression in Colorectal Cancer.
机构信息
Division of Anatomy, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland;
出版信息
In Vivo. 2022 Jan-Feb;36(1):180-188. doi: 10.21873/invivo.12689.
BACKGROUND/AIM: The role of irisin, the extracellular part of fibronectin type III domain containing 5 (FNDC5), in colorectal cancer (CRC) is unclear. The aim of this study was to investigate immunohistochemical (IHC) expression level of irisin and correlations with clinicopathological factors in patients with CRC.
MATERIALS AND METHODS
We collected 222 archived CRC samples and 26 control samples from autopsies conducted at the Department of Forensic Medicine. They were used to perform IHC reactions detecting irisin, Ki-67, minichromosome maintenance protein complex component 3 (MCM3), and urine diphosphate-galactose ceramide galactosyltransferase (UGT3) expression. The correlations with Ki-67, MCM3, and UGT3 were analyzed. Irisin expression was also evaluated in cancer cell lines by immunofluorescence reaction and western blot.
RESULTS
Irisin expression was higher in cancer cells compared to the control tissues (p<0.0001). Irisin expression was significantly higher in stage I than in stage III (p=0.013) and IV CRC (p=0.05).
CONCLUSION
The correlation between higher expression of irisin and cancer stages indicates its potential usefulness as a marker in CRC.
背景/目的：细胞外纤维连接蛋白 III 型结构域包含 5（FNDC5）的一部分鸢尾素在结直肠癌（CRC）中的作用尚不清楚。本研究旨在探讨鸢尾素在 CRC 患者中的免疫组织化学（IHC）表达水平及其与临床病理因素的相关性。
材料和方法
我们收集了 222 例存档的 CRC 样本和 26 例尸检组织样本（来自法医学系）。使用这些样本进行免疫组织化学反应，以检测鸢尾素、Ki-67、微小染色体维持蛋白复合体成分 3（MCM3）和二磷酸尿苷-半乳糖神经酰胺半乳糖基转移酶（UGT3）的表达。分析了与 Ki-67、MCM3 和 UGT3 的相关性。通过免疫荧光反应和 Western blot 评估了鸢尾素在癌细胞系中的表达。
结果
与对照组织相比，癌细胞中的鸢尾素表达更高（p<0.0001）。Irisin 表达在 I 期 CRC 中明显高于 III 期（p=0.013）和 IV 期 CRC（p=0.05）。
结论
鸢尾素表达与癌症分期之间的相关性表明其作为 CRC 标志物的潜在用途。
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